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Article

Effects of Fractionated Radiation Exposure on Vimentin Expression in Cervical Cancers: Analysis of Association with Cancer Stem Cell Response and Short-Term Prognosis

by
Irina Zamulaeva
1,*,
Olga Matchuk
1,
Elena Selivanova
1,
Liana Mkrtchian
2,
Anna Yakimova
1,
Victoria Gusarova
1,
Nikita Lipunov
1,
Liudmila Krikunova
2,
Sergey Ivanov
3,4 and
Andrey Kaprin
4,5
1
Department of Radiation Biochemistry, A. Tsyb Medical Radiological Research Center—Branch of the National Medical Research Radiological Center of the Ministry of Health of the Russian Federation, Korolev Str.-4, Obninsk 249036, Russia
2
Department of Radiation and Combined Treatment of Gynecological Diseases, A. Tsyb Medical Radiological Research Center—Branch of the National Medical Research Radiological Center of the Ministry of Health of the Russian Federation, Korolev Str.-4, Obninsk 249036, Russia
3
A. Tsyb Medical Radiological Research Center—Branch of the National Medical Research Radiological Center of the Ministry of Health of the Russian Federation, Korolev Str.-4, Obninsk 249036, Russia
4
Department of Urology and Operative Nephrology, RUDN University, Miklukho-Maklaya Str.-6, Moscow 117198, Russia
5
National Medical Research Radiological Center of the Ministry of Health of the Russian Federation, Korolev Str.-4, Obninsk 249036, Russia
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2023, 24(4), 3271; https://doi.org/10.3390/ijms24043271
Submission received: 28 November 2022 / Revised: 1 February 2023 / Accepted: 3 February 2023 / Published: 7 February 2023
(This article belongs to the Special Issue Advances in Radiation Toxicity)
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Abstract

Elucidation of the mechanisms for the response of cancer stem cells (CSCs) to radiation exposure is of considerable interest for further improvement of radio- and chemoradiotherapy of cervical cancer (CC). The aim of this work is to evaluate the effects of fractionated radiation exposure on the expression of vimentin, which is one of the end-stage markers of epithelial-mesenchymal transition (EMT), and analyze its association with CSC radiation response and short-term prognosis of CC patients. The level of vimentin expression was determined in HeLa, SiHa cell lines, and scrapings from the cervix of 46 CC patients before treatment and after irradiation at a total dose of 10 Gy using real-time polymerase chain reaction (PCR) assay, flow cytometry, and fluorescence microscopy. The number of CSCs was assessed using flow cytometry. Significant correlations were shown between vimentin expression and postradiation changes in CSC numbers in both cell lines (R = 0.88, p = 0.04 for HeLa and R = 0.91, p = 0.01 for SiHa) and cervical scrapings (R = 0.45, p = 0.008). Associations were found at the level of tendency between postradiation increase in vimentin expression and unfavorable clinical outcome 3–6 months after treatment. The results clarify some of the relationships between EMT, CSCs, and therapeutic resistance that are needed to develop new strategies for cancer treatment.
Keywords: cancer stem cells; cervical cancer; HeLa; SiHa; epithelial-mesenchymal transition; vimentin; ionizing radiation; radiosensitivity; flow cytometry cancer stem cells; cervical cancer; HeLa; SiHa; epithelial-mesenchymal transition; vimentin; ionizing radiation; radiosensitivity; flow cytometry

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MDPI and ACS Style

Zamulaeva, I.; Matchuk, O.; Selivanova, E.; Mkrtchian, L.; Yakimova, A.; Gusarova, V.; Lipunov, N.; Krikunova, L.; Ivanov, S.; Kaprin, A. Effects of Fractionated Radiation Exposure on Vimentin Expression in Cervical Cancers: Analysis of Association with Cancer Stem Cell Response and Short-Term Prognosis. Int. J. Mol. Sci. 2023, 24, 3271. https://doi.org/10.3390/ijms24043271

AMA Style

Zamulaeva I, Matchuk O, Selivanova E, Mkrtchian L, Yakimova A, Gusarova V, Lipunov N, Krikunova L, Ivanov S, Kaprin A. Effects of Fractionated Radiation Exposure on Vimentin Expression in Cervical Cancers: Analysis of Association with Cancer Stem Cell Response and Short-Term Prognosis. International Journal of Molecular Sciences. 2023; 24(4):3271. https://doi.org/10.3390/ijms24043271

Chicago/Turabian Style

Zamulaeva, Irina, Olga Matchuk, Elena Selivanova, Liana Mkrtchian, Anna Yakimova, Victoria Gusarova, Nikita Lipunov, Liudmila Krikunova, Sergey Ivanov, and Andrey Kaprin. 2023. "Effects of Fractionated Radiation Exposure on Vimentin Expression in Cervical Cancers: Analysis of Association with Cancer Stem Cell Response and Short-Term Prognosis" International Journal of Molecular Sciences 24, no. 4: 3271. https://doi.org/10.3390/ijms24043271

APA Style

Zamulaeva, I., Matchuk, O., Selivanova, E., Mkrtchian, L., Yakimova, A., Gusarova, V., Lipunov, N., Krikunova, L., Ivanov, S., & Kaprin, A. (2023). Effects of Fractionated Radiation Exposure on Vimentin Expression in Cervical Cancers: Analysis of Association with Cancer Stem Cell Response and Short-Term Prognosis. International Journal of Molecular Sciences, 24(4), 3271. https://doi.org/10.3390/ijms24043271

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