Preparation, Characterization, and Biological Properties of Hydroxyapatite from Bigeye Snapper (Priancanthus tayenus) Bone
Abstract
:1. Introduction
2. Results and Discussion
2.1. Chemical Composition of Bigeye Snapper Bone
2.2. Optimization of Hydroxyapatite Extraction Condition
2.3. Characterization of Extracted Hydroxyapatite
2.4. Hydroxyapatite Precipitation from the Extracted Solution
2.5. Cell Cytotoxicity
2.6. Adsorption of Protein on Hydroxyapatite
2.7. Desorption of Protein on Hydroxyapatite
3. Materials and Methods
3.1. Material and Preparation
3.2. Chemicals
3.3. Hydroxyapatite Extraction from Bigeye Snapper Bones
3.4. Optimization of Experimental Design
3.5. Analyzes
3.5.1. Proximate Compositions
3.5.2. Hydroxyapatite Extraction Yield
3.5.3. Color Determination
3.5.4. Calcium and Phosphorus Content
3.5.5. Fourier Transform Infrared Spectroscopy (FTIR)
3.5.6. Scanning Electron Microscope (SEM) and Energy Dispersive X-ray (EDX)
3.5.7. X-ray Diffraction Analysis (XRD)
3.6. The Precipitation of Hydroxyapatite from the Extracted Solution
3.7. In Vitro Cell Viability
3.8. Adsorption of Protein on Hydroxyapatite
3.9. Desorption of Protein from Protein-Adsorbed Hydroxyapatite
3.10. Statistical Analysis
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Treatment | X1 (min) | X2 (%) | Yield (%) | Ca ns (g/100 g) | P ns (g/100 g) | Ca/P Ratio ns | L* | a* | b* | ΔE |
---|---|---|---|---|---|---|---|---|---|---|
1 | 10 | 2.0 | 4.26 ± 0.20 e | 15.0 ± 4.87 | 7.38 ± 2.24 | 2.03 ± 0.03 | 79.8 ± 0.13 e | 4.16 ± 0.38 a | 19.3 ± 0.91 a | 31.1 ± 1.24 a |
2 | 10 | 5.0 | 10.6 ± 0.16 bc | 19.7 ± 1.58 | 9.43 ± 1.12 | 2.09 ± 0.06 | 90.3 ± 2.83 ab | 4.20 ± 1.63 a | 20.3 ± 2.18 a | 21.8 ± 1.89 c |
3 | 60 | 2.0 | 6.77 ± 0.43 d | 16.4 ± 2.44 | 8.08 ± 1.23 | 2.03 ± 0.00 | 75.2 ± 0.76 e | 3.62 ± 0.78 ab | 18.1 ± 1.33 ab | 29.0 ± 2.05 ab |
4 | 60 | 5.0 | 13.4 ± 1.30 a | 19.8 ± 3.90 | 9.55 ± 2.07 | 2.08 ± 0.03 | 91.3 ± 1.35 a | 2.84 ± 0.80 c | 16.5 ± 2.14 bc | 11.1 ± 2.39 f |
5 | 35 | 2.0 | 6.25 ± 0.11 d | 17.9 ± 0.03 | 8.65 ± 0.03 | 2.07 ± 0.00 | 76.9 ± 2.01 e | 3.11 ± 0.82 b | 18.6 ± 1.94 ab | 30.6 ± 0.67 e |
6 | 35 | 5.0 | 12.4 ± 1.03 a | 17.2 ± 2.58 | 8.28 ± 1.21 | 2.08 ± 0.00 | 89.7 ± 0.87 ab | 2.67 ± 0.29 c | 17.4 ± 1.42 b | 16.1 ± 0.43 b |
7 | 10 | 3.5 | 9.26 ± 0.15 c | 17.5 ± 1.16 | 8.30 ± 0.56 | 2.11 ± 0.00 | 87.1 ± 1.09 abc | 2.88 ± 0.25 c | 17.1 ± 1.57 b | 28.8 ± 1.57 d |
8 | 60 | 3.5 | 11.8 ± 0.71 ab | 20.1 ± 0.59 | 9.52 ± 0.39 | 2.11 ± 0.02 | 77.9 ± 2.55 de | 2.47 ± 0.23 c | 17.2 ± 0.73 b | 18.4 ± 1.31 c |
9 | 35 | 3.5 | 9.42 ± 0.12 c | 19.7 ± 0.80 | 9.27 ± 0.70 | 2.13 ± 0.05 | 85.6 ± 0.62 bc | 1.04 ± 0.35 d | 11.7 ± 2.55 d | 22.6 ± 0.28 c |
10 | 35 | 3.5 | 10.3 ± 0.15 bc | 16.2 ± 0.14 | 7.83 ± 0.30 | 2.06 ± 0.04 | 87.7 ± 0.62 abc | 1.05 ± 0.59 d | 10.8 ± 1.45 e | 22.9 ± 0.39 c |
11 | 35 | 3.5 | 10.4 ± 0.21 bc | 19.2 ± 2.24 | 9.23 ± 1.10 | 2.08 ± 0.00 | 84.4 ± 1.65 cd | 1.68 ± 0.44 d | 12.7 ± 1.49 d | 22.2 ± 2.00 c |
Responses | Quadratic Polynomial Model | R2 | p-Value |
---|---|---|---|
Yield (%) | Y1 = −5.82 + 6.01X1 + 0.05X2 − 0.002X1X2 − 0.57X12 − 0.0001X22 | 0.9827 | 0.0002 |
L* | Y2 = 81.3 + 1.59X1 + 0.05X2 − 0.012X1X2 − 0.03X12 + 0.00003X22 | 0.9000 | 0.0154 |
a* | Y3 = 6.10 − 0.77X1 − 0.02X2 + 0.008X1X2 − 0.06X12 − 0.0002X22 | 0.9741 | 0.0006 |
b* | Y4 = 17.5 + 2.44X1 + 0.004X2 + 0.01X1X2 − 0.78X12 − 0.001X22 | 0.9512 | 0.0027 |
ΔE | Y5 = 45.2 − 14.8X1 − 0.43X2 − 0.02X1X2 + 2.165X12 + 0.01X22 | 0.8772 | 0.0250 |
Ca content (g/100 g) | Y6 = 18.41 + 1.24X1 − 0.68X2 − 0.3X1X2 − 0.94X12 + 0.29X22 | 0.4770 | 0.5382 |
P content (g/100 g) | Y7 = 8.77 − 0.53X1 + 0.34X2 − 0.15X1X2 − 0.31X12 + 0.14X22 | 0.4653 | 0.5587 |
Ca/P ratio | Y8 = 2.10 + 0.02X1 − 0.002X2 − 0.003X1X2 − 0.035X12 + 0.0003X22 | 0.5782 | 0.3688 |
Response & Factors | Parameter | Predicated Value | Experimental Value | Composite Desirability | ||||
---|---|---|---|---|---|---|---|---|
Goal | Lower | Upper | Weight | Importance | ||||
X1 | Is in range | 10 | 60 | 1 | 3 | 60 | 60 | 0.908 |
X2 | Is in range | 2 | 5 | 1 | 3 | 5 | 5 | |
Yield (%) | maximize | 4.26 | 13.39 | 1 | 3 | 13.39 | 13.4 ± 1.30 | |
L* | maximize | 84.96 | 88.65 | 1 | 3 | 88.0 | 84.5 ± 2.21 | |
a* | Is in range | 1.03 | 4.20 | 1 | 3 | 1.47 | 2.84 ± 0.80 | |
b* | Is in range | 10.83 | 20.32 | 1 | 3 | 12.25 | 16.5 ± 2.14 | |
ΔE | Is in range | 8.25 | 20.39 | 1 | 3 | 16.31 | 15.6 ± 2.95 | |
Ca content (g/100 g) | none | 15.03 | 20.07 | 1 | 3 | 19.31 | 19.8 ± 3.90 | |
P content (g/100 g) | none | 7.38 | 9.55 | 1 | 3 | 9.29 | 9.55 ± 2.07 | |
Ca/P ratio | none | 2.03 | 2.13 | 1 | 3 | 2.08 | 2.08 ± 0.03 |
Treatment | Yield ns (%) | L* | a* | b* | ∆E |
---|---|---|---|---|---|
5% NaOH | 15.89 ± 1.22 | 85.84 ± 0.01 f | 2.81 ± 0.01 c | 15.10 ± 0.00 c | 16.76 ± 0.00 c |
10% NaOH | 15.93 ± 1.06 | 87.56 ± 0.01 d | 2.33 ± 0.01 e | 14.31 ± 0.02 e | 15.05 ± 0.01 e |
15% NaOH | 16.08 ± 0.30 | 86.59 ± 0.01 e | 2.56 ± 0.01 d | 14.40 ± 0.05 d | 15.78 ± 0.02 d |
20% NaOH | 16.09 ± 1.10 | 84.36 ± 0.03 h | 3.30 ± 0.00 a | 16.99 ± 0.05 b | 18.98 ± 0.04 a |
5% KOH | 15.11 ± 1.19 | 88.50 ± 0.02 b | 1.76 ± 0.00 h | 13.60 ± 0.03 g | 13.91 ± 0.03 g |
10% KOH | 16.08 ± 0.39 | 88.78 ± 0.03 a | 1.96 ± 0.01 g | 13.98 ± 0.03 f | 14.06 ± 0.02 f |
15% KOH | 16.87 ± 0.24 | 88.06 ± 0.01 c | 2.02 ± 0.01 f | 13.38 ± 0.02 h | 14.08 ± 0.02 f |
20% KOH | 17.05 ± 1.32 | 85.23 ± 0.01 g | 3.08 ± 0.01 b | 17.27 ± 0.02 a | 18.50 ± 0.02 b |
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Jindapon, N.; Klinmalai, P.; Surayot, U.; Tanadchangsaeng, N.; Pichaiaukrit, W.; Phimolsiripol, Y.; Vichasilp, C.; Wangtueai, S. Preparation, Characterization, and Biological Properties of Hydroxyapatite from Bigeye Snapper (Priancanthus tayenus) Bone. Int. J. Mol. Sci. 2023, 24, 2776. https://doi.org/10.3390/ijms24032776
Jindapon N, Klinmalai P, Surayot U, Tanadchangsaeng N, Pichaiaukrit W, Phimolsiripol Y, Vichasilp C, Wangtueai S. Preparation, Characterization, and Biological Properties of Hydroxyapatite from Bigeye Snapper (Priancanthus tayenus) Bone. International Journal of Molecular Sciences. 2023; 24(3):2776. https://doi.org/10.3390/ijms24032776
Chicago/Turabian StyleJindapon, Nunnuth, Phatthranit Klinmalai, Utoomporn Surayot, Nuttapol Tanadchangsaeng, Woradej Pichaiaukrit, Yuthana Phimolsiripol, Chaluntorn Vichasilp, and Sutee Wangtueai. 2023. "Preparation, Characterization, and Biological Properties of Hydroxyapatite from Bigeye Snapper (Priancanthus tayenus) Bone" International Journal of Molecular Sciences 24, no. 3: 2776. https://doi.org/10.3390/ijms24032776