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Article

Release of HMGB1 and Toll-like Receptors 2, 4, and 9 Signaling Are Modulated by Bifidobacterium animalis subsp. lactis BB-12 and Salmonella Typhimurium in a Gnotobiotic Piglet Model of Preterm Infants

1
Laboratory of Gnotobiology, Institute of Microbiology, Czech Academy of Sciences, 549 22 Novy Hradek, Czech Republic
2
Department of Food Science and Human Nutrition, University of Illinois, Urbana, IL 61801, USA
3
Department of Neonatology, Institute for the Care of Mother and Child, 147 00 Prague, Czech Republic
4
Department of Microbiology, Nutrition and Dietetics, Czech University of Life Sciences in Prague, 160 00 Prague, Czech Republic
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2023, 24(3), 2329; https://doi.org/10.3390/ijms24032329
Received: 17 December 2022 / Revised: 16 January 2023 / Accepted: 19 January 2023 / Published: 24 January 2023
(This article belongs to the Special Issue Advances in Gut Microbiome in Health and Disease)

Abstract

Gnotobiotic (GN) animals with defined microbiota allow us to study host–microbiota and microbiota–microbiota interferences. Preterm germ-free (GF) piglets were mono-associated with probiotic Bifidobacterium animalis subsp. lactis BB-12 (BB12) to ameliorate/prevent the consequences of infection with the Salmonella Typhimurium strain LT2 (LT2). Goblet cell density; expression of Toll-like receptors (TLRs) 2, 4, and 9; high mobility group box 1 (HMGB1); interleukin (IL)-6; and IL-12/23p40 were analyzed to evaluate the possible modulatory effect of BB12. BB12 prevented an LT2-induced decrease of goblet cell density in the colon. TLRs signaling modified by LT2 was not influenced by the previous association with BB12. The expression of HMGB1, IL-6, and IL12/23p40 in the jejunum, ileum, and colon and their levels in plasma were all decreased by BB12, but these changes were not statistically significant. In the colon, differences in HMGB1 distribution between the GF and LT2 piglet groups were observed. In conclusion, the mono-association of GF piglets with BB12 prior to LT2 infection partially ameliorated the inflammatory response to LT2 infection.
Keywords: Bifidobacterium animalis subsp. lactis BB-12; Salmonella Typhimurium; high mobility group box 1; Toll-like receptors; tight junction proteins; mucin; intestinal barrier; inflammatory cytokines; immunodeficient host Bifidobacterium animalis subsp. lactis BB-12; Salmonella Typhimurium; high mobility group box 1; Toll-like receptors; tight junction proteins; mucin; intestinal barrier; inflammatory cytokines; immunodeficient host

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MDPI and ACS Style

Splichal, I.; Donovan, S.M.; Kindlova, Z.; Stranak, Z.; Neuzil Bunesova, V.; Sinkora, M.; Polakova, K.; Valaskova, B.; Splichalova, A. Release of HMGB1 and Toll-like Receptors 2, 4, and 9 Signaling Are Modulated by Bifidobacterium animalis subsp. lactis BB-12 and Salmonella Typhimurium in a Gnotobiotic Piglet Model of Preterm Infants. Int. J. Mol. Sci. 2023, 24, 2329. https://doi.org/10.3390/ijms24032329

AMA Style

Splichal I, Donovan SM, Kindlova Z, Stranak Z, Neuzil Bunesova V, Sinkora M, Polakova K, Valaskova B, Splichalova A. Release of HMGB1 and Toll-like Receptors 2, 4, and 9 Signaling Are Modulated by Bifidobacterium animalis subsp. lactis BB-12 and Salmonella Typhimurium in a Gnotobiotic Piglet Model of Preterm Infants. International Journal of Molecular Sciences. 2023; 24(3):2329. https://doi.org/10.3390/ijms24032329

Chicago/Turabian Style

Splichal, Igor, Sharon M. Donovan, Zdislava Kindlova, Zbynek Stranak, Vera Neuzil Bunesova, Marek Sinkora, Katerina Polakova, Barbora Valaskova, and Alla Splichalova. 2023. "Release of HMGB1 and Toll-like Receptors 2, 4, and 9 Signaling Are Modulated by Bifidobacterium animalis subsp. lactis BB-12 and Salmonella Typhimurium in a Gnotobiotic Piglet Model of Preterm Infants" International Journal of Molecular Sciences 24, no. 3: 2329. https://doi.org/10.3390/ijms24032329

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