Next Article in Journal
Endometriosis of the Cervix: A Rare Clinical Case with the Possibility of Comparing the Eutopic and Ectopic Endometrium at the Cellular Level
Previous Article in Journal
Functional Characterization of the GNAT Family Histone Acetyltransferase Elp3 and GcnE in Aspergillus fumigatus
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
IJMS
Volume 24
Issue 3
10.3390/ijms24032187
ijms-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

Artificial Infestation of Sarcoptes scabiei (Acari: Sarcoptidae) in Rabbits Exhibits Progressive Pathological Changes, Apoptosis, and Keratinization in the Skin

by
Ke Guan
1,†,
Jing Xu
1,*,†,
Xiaobin Gu
1,
Ran He
1,
Yue Xie
1,
Bo Jing
1,
Xuerong Peng
2 and
Guangyou Yang
1,*
1
Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China
2
Department of Chemistry, College of Life and Basic Science, Sichuan Agricultural University, Chengdu 611130, China
*
Authors to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Int. J. Mol. Sci. 2023, 24(3), 2187; https://doi.org/10.3390/ijms24032187
Submission received: 16 December 2022 / Revised: 14 January 2023 / Accepted: 17 January 2023 / Published: 22 January 2023
(This article belongs to the Section Molecular Pathology, Diagnostics, and Therapeutics)
Browse Figures
Versions Notes

Abstract

Sarcoptes scabiei (S. scabiei) is an ectoparasite that can infest humans and 150 mammalian host species, primarily causing pruritus, crust, and alopecia. However, neither the pathological process of host skin under S. scabiei infection nor the mechanism of S. scabiei infection in regulating apoptosis and keratinization of host skin has been studied yet. In this study, a total of 56 rabbits were artificially infested with S. scabiei, and the skin samples were collected at seven different time points, including 6 h, 12 h, day 1, day 3, 1 week, 4 weeks, and 8 weeks, whereas a group of eight rabbits served as controls. We measured epidermal thickness by H&E staining, observed the skin ultrastructure by electron microscopy, and detected the degree of skin apoptosis by TUNEL staining. The level of transcription of genes related to apoptosis and keratinization was detected by quantitative real-time PCR (qRT-PCR), and the level of Bcl-2 protein expression was further detected using immunohistochemistry. Our results showed that, with increased infestation time, the epidermal layer of the rabbit skin exhibited significant thickening and keratinization, swollen mitochondria in the epidermal cells, and increased skin apoptosis. The level of caspase-1, 3, 8, 10, 14, and Bcl-2 mRNA expression was increased, whereas the level of keratin 1 and 5 was decreased after S. scabiei infestation. In conclusion, S. scabiei infestation causes thickening of the epidermis, which may be related to apoptosis-induced proliferation and skin keratinization.
Keywords: Sarcoptes scabiei; pathological changes; rabbits; skin; apoptosis; keratinization Sarcoptes scabiei; pathological changes; rabbits; skin; apoptosis; keratinization

Share and Cite

MDPI and ACS Style

Guan, K.; Xu, J.; Gu, X.; He, R.; Xie, Y.; Jing, B.; Peng, X.; Yang, G. Artificial Infestation of Sarcoptes scabiei (Acari: Sarcoptidae) in Rabbits Exhibits Progressive Pathological Changes, Apoptosis, and Keratinization in the Skin. Int. J. Mol. Sci. 2023, 24, 2187. https://doi.org/10.3390/ijms24032187

AMA Style

Guan K, Xu J, Gu X, He R, Xie Y, Jing B, Peng X, Yang G. Artificial Infestation of Sarcoptes scabiei (Acari: Sarcoptidae) in Rabbits Exhibits Progressive Pathological Changes, Apoptosis, and Keratinization in the Skin. International Journal of Molecular Sciences. 2023; 24(3):2187. https://doi.org/10.3390/ijms24032187

Chicago/Turabian Style

Guan, Ke, Jing Xu, Xiaobin Gu, Ran He, Yue Xie, Bo Jing, Xuerong Peng, and Guangyou Yang. 2023. "Artificial Infestation of Sarcoptes scabiei (Acari: Sarcoptidae) in Rabbits Exhibits Progressive Pathological Changes, Apoptosis, and Keratinization in the Skin" International Journal of Molecular Sciences 24, no. 3: 2187. https://doi.org/10.3390/ijms24032187

APA Style

Guan, K., Xu, J., Gu, X., He, R., Xie, Y., Jing, B., Peng, X., & Yang, G. (2023). Artificial Infestation of Sarcoptes scabiei (Acari: Sarcoptidae) in Rabbits Exhibits Progressive Pathological Changes, Apoptosis, and Keratinization in the Skin. International Journal of Molecular Sciences, 24(3), 2187. https://doi.org/10.3390/ijms24032187

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop