Mesenchymal Stromal Cells from Perinatal Tissues as an Alternative for Ex Vivo Expansion of Hematopoietic Progenitor and Stem Cells from Umbilical Cord Blood
Abstract
:1. Introduction
2. Results
2.1. Isolation and Characterization of Mesenchymal Stromal Cells from Bone Marrow and Perinatal Tissues
2.2. Definition of Culture Conditions for Hematopoietic Stem and Progenitor Cell Expansion
2.3. Evaluation of the Supportive Capacity of Perinatal Mesenchymal Stromal Cells in Hematopoietic Stem- and Progenitor-Cell Proliferation
2.4. Evaluation of the Migratory Profile of Hematopoietic Stem and Progenitor Cells in Co-Culture with Mesenchymal Stromal Cells from Perinatal Tissues
2.5. Determination of the Expression of Primitive and Lineage-Committed Markers in Hematopoietic Stem Progenitor Cells Cultured with Mesenchymal Stromal Cells from Perinatal Tissues
2.6. Evaluation of the Clonogenic Potential of Hematopoietic Stem Progenitor Cells in Co-Culture with Mesenchymal Stromal Cells from Perinatal Tissues
2.7. Evaluation of the Secretory Profile of Hematopoietic Stem Cells in Co-Culture with Mesenchymal Stromal Cells from Perinatal Tissues
2.8. Principal Component Analysis for Assessing the Impact on Functionality Variables of HSPCs during Co-Culture with Perinatal-Tissue-Derived MSCs
3. Discussion
4. Materials and Methods
4.1. Donor Selection
4.2. Isolation of Cell Lines from BM MSCs
4.3. Isolation of Cell Lines from WJ MSCs
4.4. Isolation of Cell Lines from UCB MSCs
4.5. Immunophenotypic Characterization of MSCs
4.6. CD34+ HSPCs Immunomagnetic Isolation
4.7. Irradiation of MSCs
4.8. Co-Culture of MSCs with HSPCs
4.9. Assessment of Proliferation Capacity
4.10. Evaluation of Migration Capacity
4.11. Determination of Differentiation Capacity
4.12. Determination of Clonogenic Potential
4.13. Identification and Quantification of Cytokines in MSC Monoculture and Co-Culture Conditioned Media
4.14. Variable Correlation Analysis
4.15. Data Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Bonilla, X.; Lara, A.M.; Llano-León, M.; López-González, D.A.; Hernández-Mejía, D.G.; Bustos, R.H.; Camacho-Rodríguez, B.; Perdomo-Arciniegas, A.-M. Mesenchymal Stromal Cells from Perinatal Tissues as an Alternative for Ex Vivo Expansion of Hematopoietic Progenitor and Stem Cells from Umbilical Cord Blood. Int. J. Mol. Sci. 2023, 24, 15544. https://doi.org/10.3390/ijms242115544
Bonilla X, Lara AM, Llano-León M, López-González DA, Hernández-Mejía DG, Bustos RH, Camacho-Rodríguez B, Perdomo-Arciniegas A-M. Mesenchymal Stromal Cells from Perinatal Tissues as an Alternative for Ex Vivo Expansion of Hematopoietic Progenitor and Stem Cells from Umbilical Cord Blood. International Journal of Molecular Sciences. 2023; 24(21):15544. https://doi.org/10.3390/ijms242115544
Chicago/Turabian StyleBonilla, Ximena, Ana Milena Lara, Manuela Llano-León, David A. López-González, David G. Hernández-Mejía, Rosa Helena Bustos, Bernardo Camacho-Rodríguez, and Ana-María Perdomo-Arciniegas. 2023. "Mesenchymal Stromal Cells from Perinatal Tissues as an Alternative for Ex Vivo Expansion of Hematopoietic Progenitor and Stem Cells from Umbilical Cord Blood" International Journal of Molecular Sciences 24, no. 21: 15544. https://doi.org/10.3390/ijms242115544