Potential Antitumor Effect of α-Mangostin against Rat Mammary Gland Tumors Induced by LA7 Cells
Abstract
:1. Introduction
2. Results
2.1. Tumor Development
2.2. Body Weight, Tumor Size, and Tumor Volume
2.3. Blood Biochemical Parameters
2.4. Histopathology
2.5. Apoptosis
2.6. Antioxidant Activity
2.7. Immunohistochemistry of PCNA and p53
3. Discussion
4. Materials and Methods
4.1. Extraction and Isolation of AM from C. arborescens
4.2. Identification of AM
4.3. Chemicals, Reagents, and Cell Culture
4.4. Ethical Issues
4.5. Cell Preparation
4.6. Induction of Mammary Gland Tumors
4.7. Experimental Design and Animal Treatment
4.8. Serum Biochemical Parameters
4.9. Biochemical Parameters of Mammary and Liver Tissues
4.9.1. Estimation of Superoxide Dismutase (SOD) Activity
4.9.2. Inhibition of LPx Formation
4.9.3. Estimation of Glutathione Peroxidase GPx Activity
4.9.4. Estimation of CAT Activity
4.10. Macroscopic and Microscopic Analysis
4.11. TUNEL Assay
4.12. Immunohistochemistry Examination
4.13. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Group | Treatment Groups | Body Weight (g) | Tumor Volume (mm3) | Reduction of Tumor Percentage (%) |
---|---|---|---|---|
I. | NC | 220.7 ± 2.4 * | 0 | 0 |
II. | MTC | 183.25 ± 3.31 | 423 ± 71.2 | 0 |
III. | MT + AM 30 mg/kg LD | 199.13 ± 2.8 * | 185 ± 12.8 * | 56.3% * |
IV. | MT + AM 60 mg/kg HD | 205.25 ± 2 * | 121 ± 19.3 * | 71.4% * |
V. | MT + TAM | 209.88 ± 1.7 * | 90 ± 27 * | 78.7% * |
VI. | AM 60 mg/kg | 220.1 ± 0.5 * | 0 | 0 |
Group | Treatment Group | Serum Biomarkers | ||||
---|---|---|---|---|---|---|
CEA (ng/mL) | CA15-3 (ng/mL) | ALT (U/L) | ALP (U/L) | LDH (U/L) | ||
I | NC | 3.1 ± 0.9 * | 12.2 ± 4.0 * | 45.0 ± 3.4 * | 68.0 ± 7.3 * | 612 ± 201.34 * |
II | MTC | 7.3 ± 2.3 | 48.0 ± 12.5 | 95.4 ± 16.1 | 453 ± 24 | 4562.0 ± 850 |
III | MT + AM 30 mg/kg LD | 4.6 ± 1.2 * | 32.6 ± 9.4 * | 56.0 ± 14.8 * | 224.0 ± 5.5 * | 2163.8 ± 73.1 * |
IV | MT + AM 60 mg/kg HD | 3.5 ± 0.8 * | 27.3 ± 4.6 * | 55.5 ± 14.9 * | 211.3 ± 4.1 * | 2189.7 ± 114 * |
V | MT + TAM | 3.2 ± 1.04 * | 26.2 ± 6.9 * | 66.5 ± 16.5 * | 199.2 ± 5.2 * | 2200 ± 70 * |
VI | AM 60 mg/kg | 3.1 ± 1.02 * | 12.3 ± 4.0 * | 45.0 ± 4.2 * | 68.0 ± 16.2 * | 614 ± 17.15 * |
Group | Treatment Group | Tubule | Mitotic Figure | Nuclear Pleomorphism | Grade | Total Score |
---|---|---|---|---|---|---|
I | NC | 100% tubular structure | No mitotic figure | Uniform nuclear morphology | Grade I | 3 ± 0.0 * |
II | MTC | 5% tubular formation | High mitotic figure more than 10 in high power per 10 magnification field | Moderate nuclear pleomorphism | Grade III | 8 ± 0.5 |
III | MT + AM 30 mg/kg LD | 20% tubular formation | Moderate mitotic figures | Several variation in nuclear sizes | Grade II | 6 ± 0.2 * |
IV | MT + AM 60 mg/kg HD | 50% tubular formation | Moderate mitotic figure | Moderate nuclear pleomorphism | Grade I | 5 ± 0.3 * |
V | MT + TAM | 30% tubular formation | Low mitotic figure | Few nuclear pleomorphisms | Grade I | 4 ± 0.1 * |
VI | 60 mg/kg AM | 95% tubular structure | No mitotic figure | Uniform nuclear morphology | Grade I | 3 ± 0.1 * |
Group | Treatment Groups | SOD (U/mg Protein) | CAT (µmol/mg Ptotein) | GPX (µg/mg Protein) | LPx (nmol/mg Protein) |
---|---|---|---|---|---|
I | NC | 8.42 ± 1.12 * | 62.32 ± 2.15 * | 7.24 ± 1.56 * | 1.21 ± 0.27 * |
II | MTC | 5.00 ± 1.7 | 39.61 ± 4.21 | 4.00 ± 0.23 | 3.13 ± 1.25 |
III | MT + AM 30 mg/kg LD | 6.20 ± 0.47 * | 56.30 ± 3.43 * | 5.02 ± 0.93 * | 2.12 ± 0.64 * |
IV | MT + AM 60 mg/kg HD | 7.24 ± 2.1 * | 59.40 ± 3.23 * | 5.93 ± 1.2 * | 1.12 ± 0.32 * |
V | MT + TAM | 7.29 ± 1.23 * | 61.13 ± 3.28 * | 6.56 ± 0.31 * | 1.18 ± 0.61 * |
VI | 60 mg/kg AM | 8.43 ± 2.11 * | 62.10 ± 2.27 * | 7.22 ± 0.21 * | 1.21 ± 0.11 * |
Group | Treatment Groups | SOD (U/mg Protein) | CAT (µmol/mg Protein) | GPX (µg/mg Protein) | LPX (nmol/mg Protein) |
---|---|---|---|---|---|
I | NC | 6.32 ± 1.91 * | 57.55 ± 1.33 * | 5.13 ± 1.22 * | 1.81 ± 0.36 * |
II | MTC | 2.56 ± 0.95 | 23.17 ± 16.1 | 2.27 ± 0.06 | 4.19 ± 0.13 |
III | MT + AM 30 mg/kg LD | 4.29 ± 0.08 * | 48.73 ± 0.15 * | 3.81 ± 0.09 * | 3.02 ± 0.02 * |
IV | MT + AM 60 mg/kg HD | 5.24 ± 2.1 * | 59.40 ± 3.23 * | 4.41 ± 1.4 * | 2.21 ± 0.41 * |
V | MT + TAM | 5.29 ± 0.08 * | 56.27 ± 0.12 * | 4.83 ± 0.26 * | 2.02 ± 0.17 * |
VI | 60 mg/kg AM | 6.31 ± 0.01 * | 57.58 ± 1.27 * | 5.13 ± 1.15 * | 1.81 ± 0.25 * |
Group | PCNA-Immunopositive Cells (%) | p53-Immunopositive Cells (%) |
---|---|---|
NC | 0.00 ± 0.00 | 5.10 ± 0.96 |
MTC | 73.76 ± 22.90 a | 1.50 ± 0.42 a |
MT + AM-LD | 41.35 ± 12.67 a,b | 3.7 ± 1.13 a,b |
MT + AM-HD | 32.56 ± 9.85 a,b | 4.20 ± 0.91 a,b |
MT + TAM | 30.89 ± 10.12 a,b | 4.50 ± 1.22 a,b |
AM-HD | 0.00 ± 0.00 Ns,b | 5.0 ± 1.28 Ns,b |
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Ibrahim, M.Y.; Hashim, N.M.; Omer, F.A.A.; Abubakar, M.S.; Mohammed, H.A.; Salama, S.M.; Jayash, S.N. Potential Antitumor Effect of α-Mangostin against Rat Mammary Gland Tumors Induced by LA7 Cells. Int. J. Mol. Sci. 2023, 24, 10283. https://doi.org/10.3390/ijms241210283
Ibrahim MY, Hashim NM, Omer FAA, Abubakar MS, Mohammed HA, Salama SM, Jayash SN. Potential Antitumor Effect of α-Mangostin against Rat Mammary Gland Tumors Induced by LA7 Cells. International Journal of Molecular Sciences. 2023; 24(12):10283. https://doi.org/10.3390/ijms241210283
Chicago/Turabian StyleIbrahim, Mohamed Yousif, Najihah Mohd Hashim, Fatima Abdelmutaal Ahmed Omer, Muhammad Salisu Abubakar, Hoyam Adam Mohammed, Suzy Munir Salama, and Soher Nagi Jayash. 2023. "Potential Antitumor Effect of α-Mangostin against Rat Mammary Gland Tumors Induced by LA7 Cells" International Journal of Molecular Sciences 24, no. 12: 10283. https://doi.org/10.3390/ijms241210283