Characterization and Biological Activities of Melanin from the Medicinal Fungi Ophiocordyceps sinensis
Abstract
:1. Introduction
2. Results
2.1. Induction Conditions of Melanin
2.2. Elemental Analysis
2.3. Spectral Characterization
2.4. Py-GCMS Analysis
2.5. Ability to Chelate Metal Ions
2.6. UV-Blocking Activity
2.7. Antioxidant Activity
3. Discussion
4. Materials and Methods
4.1. Strain Cultivation and Melanin Extraction
4.2. Elemental Analysis
4.3. Spectroscopy Analysis
4.3.1. UV-Visible Absorption Spectrum
4.3.2. Fourier Transform Infrared (FTIR)
4.3.3. Electron Paramagnetic Resonance (EPR)
4.4. Pyrolysis Gas Chromatography and Mass Spectrometry (Py-GCMS)
4.5. Biological Activity
4.5.1. Ability to Chelate Metal Ions
4.5.2. UV-Blocking
4.5.3. Free-Radical-Scavenging Ability
4.5.4. Antioxidant Activity on Cells
4.6. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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C | H | O | N | S | C/N | C/H | C/O | |
---|---|---|---|---|---|---|---|---|
O. sinensis | 50.59 | 6.18 | 33.90 | 8.19 | 1.20 | 7.21 | 9.55 | 1.99 |
Eumelanin * [21] | 56.45 | 3.15 | 31.82 | 8.49 | 0.09 | 7.76 | 20.91 | 2.37 |
Phaeomelanin * [21] | 46.24 | 4.46 | 30.16 | 9.36 | 9.78 | 5.76 | 12.10 | 2.04 |
Inonotus hispidus [9] | 43.67 | 6.25 | 46.58 | 3.5 | 0 | 14.56 | 8.15 | 1.25 |
Ganoderma lucidum [3] | 54.20 | 6.24 | 33.35 | 5.14 | 1.07 | 12.30 | 10.13 | 2.17 |
Phoma sp. RDSE17 [22] | 56.71 | 5.15 | 37.52 | 0.62 | 0 | 106.71 | 12.85 | 2.02 |
Auricularia auricula [23] | 41.18 | 5.56 | 51.60 | 1.66 | 0 | 28.94 | 8.64 | 1.06 |
Termitomyces albuminosus [24] | 54.68 | 3.54 | 26.92 | 2.49 | 12.36 | 25.60 | 18.00 | 2.71 |
Boletus griseus [25] | 56.38 | 5.86 | 28.04 | 6.17 | 2.44 | 10.66 | 11.22 | 2.68 |
Num. | RT (min) | Area (%) | Structure | Num. | RT (min) | Area (%) | Structure |
---|---|---|---|---|---|---|---|
1 | 1.389 | 29.3 | 13 | 3.376 | 2.86 | ||
2 | 1.448 | 15.29 | 14 | 5.607 | 1.12 | ||
3 | 1.554 | 16.04 | 15 | 7.098 | 0.19 | ||
4 | 1.635 | 0.93 | 16 | 8.712 | 0.34 | ||
5 | 1.708 | 2.45 | 17 | 12.022 | 0.42 | ||
6 | 1.742 | 1.46 | 18 | 12.339 | 3.18 | ||
7 | 1.943 | 1.03 | 19 | 12.419 | 0.65 | ||
8 | 1.991 | 0.81 | 20 | 13.287 | 10.24 | ||
9 | 2.094 | 1.57 | 21 | 13.4 | 0.83 | ||
10 | 2.23 | 1.09 | 22 | 14.158 | 1.24 | ||
11 | 2.537 | 3.51 | 23 | 14.259 | 1.18 | ||
12 | 2.758 | 2.87 | 24 | 17.467 | 1.39 |
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Tong, C.; Luo, J.; Xie, C.; Wei, J.; Pan, G.; Zhou, Z.; Li, C. Characterization and Biological Activities of Melanin from the Medicinal Fungi Ophiocordyceps sinensis. Int. J. Mol. Sci. 2023, 24, 10282. https://doi.org/10.3390/ijms241210282
Tong C, Luo J, Xie C, Wei J, Pan G, Zhou Z, Li C. Characterization and Biological Activities of Melanin from the Medicinal Fungi Ophiocordyceps sinensis. International Journal of Molecular Sciences. 2023; 24(12):10282. https://doi.org/10.3390/ijms241210282
Chicago/Turabian StyleTong, Chaoqun, Jian Luo, Chaolu Xie, Junhong Wei, Guoqing Pan, Zeyang Zhou, and Chunfeng Li. 2023. "Characterization and Biological Activities of Melanin from the Medicinal Fungi Ophiocordyceps sinensis" International Journal of Molecular Sciences 24, no. 12: 10282. https://doi.org/10.3390/ijms241210282