Genome-Wide Analysis of Barley bHLH Transcription Factors and the Functional Characterization of HvbHLH56 in Low Nitrogen Tolerance in Arabidopsis
Abstract
:1. Introduction
2. Results
2.1. Identification of bHLH Family Genes in Barley
2.2. Multiple Sequence Alignment of HvbHLHs
2.3. Motifs and Exon/Intron Structures Analysis of HvbHLHs
2.4. Chromosomal Distribution and Gene Duplication of HvbHLH Genes
2.5. Function Prediction of HvbHLHs Based on Phylogenetic Analysis
2.6. Stress-Related Cis-Elements in the Promoter of HvbHLH Genes
2.7. Expression Profiles of HvbHLHs under Low Nitrogen Stress
2.8. HvbHLH56-Enhanced Tolerance to Low Nitrogen Stress in Transgenic Arabidopsis
3. Discussion
4. Materials and Methods
4.1. Identification of bHLH Genes in Barley
4.2. Chromosomal Location and Gene Duplication
4.3. Phylogenetic Analysis
4.4. Exon/Intron Structure and Motif Analysis
4.5. Cis-Elements in Promoter Regions of HvbHLHs
4.6. Plant Materials and Stress Treatments
4.7. Transcriptome Analysis of HvbHLH Genes in Response to LN Stress
4.8. The Dynamic Expression of HvbHLH Genes by Real-Time PCR in Response to LN Stress
4.9. Expression Pattern Analysis of HvbHLH Genes in Tissues
4.10. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
Abbreviations
References
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Quan, X.; Meng, C.; Zhang, N.; Liang, X.; Li, J.; Li, H.; He, W. Genome-Wide Analysis of Barley bHLH Transcription Factors and the Functional Characterization of HvbHLH56 in Low Nitrogen Tolerance in Arabidopsis. Int. J. Mol. Sci. 2023, 24, 9740. https://doi.org/10.3390/ijms24119740
Quan X, Meng C, Zhang N, Liang X, Li J, Li H, He W. Genome-Wide Analysis of Barley bHLH Transcription Factors and the Functional Characterization of HvbHLH56 in Low Nitrogen Tolerance in Arabidopsis. International Journal of Molecular Sciences. 2023; 24(11):9740. https://doi.org/10.3390/ijms24119740
Chicago/Turabian StyleQuan, Xiaoyan, Chen Meng, Ning Zhang, Xiaoli Liang, Jialin Li, Hongmei Li, and Wenxing He. 2023. "Genome-Wide Analysis of Barley bHLH Transcription Factors and the Functional Characterization of HvbHLH56 in Low Nitrogen Tolerance in Arabidopsis" International Journal of Molecular Sciences 24, no. 11: 9740. https://doi.org/10.3390/ijms24119740