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Article

Identification, Characterization and Expression Profiling of the RS Gene Family during the Withering Process of White Tea in the Tea Plant (Camellia sinensis) Reveal the Transcriptional Regulation of CsRS8

by
Tao Wang
1,
Yiqing Wang
1,
Jiamin Zhao
1,
Jiumei Kong
1,
Lingzhi Zhang
1,
Siyu Qi
1,
Jiajia Chen
1,
Zhidan Chen
2,
Wen Zeng
1,* and
Weijiang Sun
1,*
1
College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, China
2
Anxi College of Tea Science, Fujian Agriculture and Forestry University, Quanzhou 362000, China
*
Authors to whom correspondence should be addressed.
Int. J. Mol. Sci. 2023, 24(1), 202; https://doi.org/10.3390/ijms24010202
Submission received: 25 November 2022 / Revised: 17 December 2022 / Accepted: 18 December 2022 / Published: 22 December 2022
(This article belongs to the Special Issue Regulatory Mechanism and Network of Abiotic Stress-Response in Plants)
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Abstract

Raffinose synthetase (RS) is a key enzyme in the process of raffinose (Raf) synthesis and is involved in plant development and stress responses through regulating Raf content. As a sweetener, Raf makes an important contribution to the sweet taste of white tea. However, studies on the identification, analysis and transcriptional regulation of CsRSs (Camellia sinensis RS genes) are still lacking. In this study, nine CsRSs were identified from the tea plant (Camellia sinensis) genome database. The CsRSs were classified into five groups in the phylogenetic tree. Expression level analysis showed that the CsRSs varied in different parts of the tea plant. Transcriptome data showed that CsRSs could respond to persistent drought and cold acclimation. Except for CsRS5 and CsRS9, the expression pattern of all CsRSs increased at 12 h and decreased at 30 h during the withering process of white tea, consistent with the change trend of the Raf content. Furthermore, combining yeast one-hybrid assays with expression analysis, we found that CsDBB could potentially regulate the expression of CsRS8. Our results provide a new perspective for further research into the characterization of CsRS genes and the formation of the white tea flavour.
Keywords: Camellia sinensis; raffinose synthase; white tea; expression pattern; CsRS8; transcriptional regulation Camellia sinensis; raffinose synthase; white tea; expression pattern; CsRS8; transcriptional regulation

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MDPI and ACS Style

Wang, T.; Wang, Y.; Zhao, J.; Kong, J.; Zhang, L.; Qi, S.; Chen, J.; Chen, Z.; Zeng, W.; Sun, W. Identification, Characterization and Expression Profiling of the RS Gene Family during the Withering Process of White Tea in the Tea Plant (Camellia sinensis) Reveal the Transcriptional Regulation of CsRS8. Int. J. Mol. Sci. 2023, 24, 202. https://doi.org/10.3390/ijms24010202

AMA Style

Wang T, Wang Y, Zhao J, Kong J, Zhang L, Qi S, Chen J, Chen Z, Zeng W, Sun W. Identification, Characterization and Expression Profiling of the RS Gene Family during the Withering Process of White Tea in the Tea Plant (Camellia sinensis) Reveal the Transcriptional Regulation of CsRS8. International Journal of Molecular Sciences. 2023; 24(1):202. https://doi.org/10.3390/ijms24010202

Chicago/Turabian Style

Wang, Tao, Yiqing Wang, Jiamin Zhao, Jiumei Kong, Lingzhi Zhang, Siyu Qi, Jiajia Chen, Zhidan Chen, Wen Zeng, and Weijiang Sun. 2023. "Identification, Characterization and Expression Profiling of the RS Gene Family during the Withering Process of White Tea in the Tea Plant (Camellia sinensis) Reveal the Transcriptional Regulation of CsRS8" International Journal of Molecular Sciences 24, no. 1: 202. https://doi.org/10.3390/ijms24010202

APA Style

Wang, T., Wang, Y., Zhao, J., Kong, J., Zhang, L., Qi, S., Chen, J., Chen, Z., Zeng, W., & Sun, W. (2023). Identification, Characterization and Expression Profiling of the RS Gene Family during the Withering Process of White Tea in the Tea Plant (Camellia sinensis) Reveal the Transcriptional Regulation of CsRS8. International Journal of Molecular Sciences, 24(1), 202. https://doi.org/10.3390/ijms24010202

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