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Article

Structure-Functional Characteristics of the Svx Protein—The Virulence Factor of the Phytopathogenic Bacterium Pectobacterium atrosepticum

by
Natalia Tendiuk
1,
Tatiana Konnova
1,
Olga Petrova
1,
Elena Osipova
1,
Timur Mukhametzyanov
2,
Olga Makshakova
1 and
Vladimir Gorshkov
1,2,*
1
Kazan Institute of Biochemistry and Biophysics, FRC Kazan Scientific Center of RAS, 420111 Kazan, Russia
2
Kazan Federal University, 420008 Kazan, Russia
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2022, 23(13), 6914; https://doi.org/10.3390/ijms23136914
Submission received: 20 May 2022 / Revised: 17 June 2022 / Accepted: 20 June 2022 / Published: 21 June 2022
(This article belongs to the Special Issue Recent Advances in Protein-Protein Interactions)
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Abstract

The Svx proteins are virulence factors of phytopathogenic bacteria of the Pectobacterium genus. The specific functions of these proteins are unknown. Here we show that most of the phytopathogenic species of Pectobacterium, Dickeya, and Xanthomonas genera have genes encoding Svx proteins, as well as some plant-non-associated species of different bacterial genera. As such, the Svx-like proteins of phytopathogenic species form a distinct clade, pointing to the directed evolution of these proteins to provide effective interactions with plants. To get a better insight into the structure and functions of the Svx proteins, we analyzed the Svx of Pectobacterium atrosepticum (Pba)—an extracellular virulence factor secreted into the host plant cell wall (PCW). Using in silico analyses and by obtaining and analyzing the recombinant Pba Svx and its mutant forms, we showed that this protein was a gluzincin metallopeptidase. The 3D structure model of the Pba Svx was built and benchmarked against the experimental overall secondary structure content. Structure-based substrate specificity analysis using molecular docking revealed that the Pba Svx substrate-binding pocket might accept α-glycosylated proteins represented in the PCW by extensins—proteins that strengthen the PCW. Thus, these results elucidate the way in which the Pba Svx may contribute to the Pba virulence.
Keywords: gluzincin extracellular metallopeptidases; molecular docking; Pectobacterium; plant-pathogen interactions; protein structure; Svx proteins; virulence factors gluzincin extracellular metallopeptidases; molecular docking; Pectobacterium; plant-pathogen interactions; protein structure; Svx proteins; virulence factors

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MDPI and ACS Style

Tendiuk, N.; Konnova, T.; Petrova, O.; Osipova, E.; Mukhametzyanov, T.; Makshakova, O.; Gorshkov, V. Structure-Functional Characteristics of the Svx Protein—The Virulence Factor of the Phytopathogenic Bacterium Pectobacterium atrosepticum. Int. J. Mol. Sci. 2022, 23, 6914. https://doi.org/10.3390/ijms23136914

AMA Style

Tendiuk N, Konnova T, Petrova O, Osipova E, Mukhametzyanov T, Makshakova O, Gorshkov V. Structure-Functional Characteristics of the Svx Protein—The Virulence Factor of the Phytopathogenic Bacterium Pectobacterium atrosepticum. International Journal of Molecular Sciences. 2022; 23(13):6914. https://doi.org/10.3390/ijms23136914

Chicago/Turabian Style

Tendiuk, Natalia, Tatiana Konnova, Olga Petrova, Elena Osipova, Timur Mukhametzyanov, Olga Makshakova, and Vladimir Gorshkov. 2022. "Structure-Functional Characteristics of the Svx Protein—The Virulence Factor of the Phytopathogenic Bacterium Pectobacterium atrosepticum" International Journal of Molecular Sciences 23, no. 13: 6914. https://doi.org/10.3390/ijms23136914

APA Style

Tendiuk, N., Konnova, T., Petrova, O., Osipova, E., Mukhametzyanov, T., Makshakova, O., & Gorshkov, V. (2022). Structure-Functional Characteristics of the Svx Protein—The Virulence Factor of the Phytopathogenic Bacterium Pectobacterium atrosepticum. International Journal of Molecular Sciences, 23(13), 6914. https://doi.org/10.3390/ijms23136914

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