Excitatory Effects of Calcitonin Gene-Related Peptide (CGRP) on Superficial Sp5C Neurons in Mouse Medullary Slices
Abstract
:1. Introduction
2. Results
2.1. Firing Patterns of Sp5C Laminae I/II Neurons
2.2. CGRP Excites Tonic Spiking Neurons in Sp5C Laminae I/II
2.3. CGRP Facilitates Excitatory Synaptic Inputs onto Laminae I/II Neurons
2.4. CGRP Receptor Immunofluorescence in Sp5C Laminae I/II
3. Discussion
3.1. Classification of Sp5C Laminae I/II Neurons
3.2. CGRP Promotes Neuronal Excitability in Sp5C Superficial Laminae
3.3. CGRP Enhances Glutamatergic Transmission in Sp5C Superficial Laminae
3.4. Pre- or Postsynaptic Mechanisms of CGRP?
4. Materials and Methods
4.1. Animals and Trigeminal Nucleus Slice Preparation
4.2. Electrophysiological Recordings
4.3. Method for Biocytin Labeling
4.4. Immunohistochemistry for CGRP Receptors
4.5. Statistical Analysis
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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KA + Picrotoxin + | Strychnine | |||||
---|---|---|---|---|---|---|
Tonic spiking cells | Control (n = 8) | CGRP (100 nM) | Control (n = 7) | CGRP (100 nM) | Control (n = 9) | CGRP (500 nM) |
Resting membrane potential (RMP; mV) | −69.08 ± 1.92 | - | −66.30 ± 3.27 | - | −67.42 ± 1.53 | - |
Cm (pF) | 58.78 ± 7.92 | - | 53.33 ± 11.07 | - | 52.50 ± 6.50 | - |
# of APs per pulse | 6.13 ± 0.52 | 11.75 ± 1.59 ** | 7.42 ± 0.90 | 8.71 ± 1.34 | 6.55 ± 0.75 | 9.89 ± 1.31 ** |
Time to 1st AP (ms) | 41.80 ± 9.22 | 22.10 ± 5.92 * | 41.93 ± 9.19 | 35.33 ± 8.36 * | 40.98 ± 4.49 | 27.94 ± 4.49 ** |
AP amplitude (mV) | 66.20 ± 3.51 | 66.80 ± 2.68 | 62.50 ± 3.39 | 60.86 ± 3.77 | 62.04 ± 2.71 | 62.29 ± 2.55 |
AP threshold (mV) | −45.56 ± 1.29 | −47.92 ± 1.53 * | −45.56 ± 1.40 | −46.48 ± 1.66 * | −46.41 ± 1.08 | −48.14 ± 1.26 ** |
Maximal rising slope (mV/ms) | 233.80 ± 24.50 | 237.20 ± 20.11 | 200.58 ± 26.61 | 192.33 ± 25.17 | 203.06 ± 20.92 | 202.79 ± 17.06 |
1/2 width of AP (ms) | 0.78 ± 0.09 | 0.83 ± 0.12 | 1.04 ± 0.13 | 1.07 ± 0.12 | 0.92 ± 0.09 | 0.93 ± 0.08 |
Afterhyperpolarization (AHP; mV) | 13.50 ± 2.21 | 12.50 ± 2.20 * | 11.59 ± 1.20 | 10.28 ± 1.23 * | 14.95 ± 1.76 | 13.59 ± 1.91 ** |
RN (MΩ) | 310.17 ± 49.18 | 376.33 ± 54.10 ** | 413.00 ± 94.90 | 570.00 ± 147.11 * | 421.57 ± 42.27 | 535.00 ± 62.24 * |
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Zheng, F.; Nixdorf-Bergweiler, B.E.; van Brederode, J.; Alzheimer, C.; Messlinger, K. Excitatory Effects of Calcitonin Gene-Related Peptide (CGRP) on Superficial Sp5C Neurons in Mouse Medullary Slices. Int. J. Mol. Sci. 2021, 22, 3794. https://doi.org/10.3390/ijms22073794
Zheng F, Nixdorf-Bergweiler BE, van Brederode J, Alzheimer C, Messlinger K. Excitatory Effects of Calcitonin Gene-Related Peptide (CGRP) on Superficial Sp5C Neurons in Mouse Medullary Slices. International Journal of Molecular Sciences. 2021; 22(7):3794. https://doi.org/10.3390/ijms22073794
Chicago/Turabian StyleZheng, Fang, Barbara E. Nixdorf-Bergweiler, Johannes van Brederode, Christian Alzheimer, and Karl Messlinger. 2021. "Excitatory Effects of Calcitonin Gene-Related Peptide (CGRP) on Superficial Sp5C Neurons in Mouse Medullary Slices" International Journal of Molecular Sciences 22, no. 7: 3794. https://doi.org/10.3390/ijms22073794