Next Article in Journal
Flavonoids: Nutraceuticals for Rheumatic Diseases via Targeting of Inflammasome Activation
Next Article in Special Issue
Ionic Silicon Protects Oxidative Damage and Promotes Skeletal Muscle Cell Regeneration
Previous Article in Journal
Left Axis Deviation in Brugada Syndrome: Vectorcardiographic Evaluation during Ajmaline Provocation Testing Reveals Additional Depolarization Abnormalities
Previous Article in Special Issue
Surface Modification of PLLA, PTFE and PVDF with Extreme Ultraviolet (EUV) to Enhance Cell Adhesion
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
IJMS
Volume 22
Issue 2
10.3390/ijms22020485
ijms-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

Application of Raman Spectroscopic Imaging to Assess the Structural Changes at Cell-Scaffold Interface

by
Grzegorz Kalisz
1,
Agata Przekora
2,*,
Paulina Kazimierczak
2,
Barbara Gieroba
1,
Michal Jedrek
1,3,
Wojciech Grudzinski
4,
Wieslaw I. Gruszecki
4,
Grazyna Ginalska
2 and
Anna Sroka-Bartnicka
1,5,*
1
Department of Biopharmacy, Medical University of Lublin, Chodzki 4a, 20-093 Lublin, Poland
2
Department of Biochemistry and Biotechnology, Medical University of Lublin, Chodzki 1, 20-093 Lublin, Poland
3
Collegium Medicum, Cardinal Stefan Wyszynski University in Warsaw, Dewajtis 5, 01-815 Warsaw, Poland
4
Department of Biophysics, Institute of Physics, Maria Curie-Sklodowska University, pl. Marii Curie-Sklodowskiej 1, 20-031 Lublin, Poland
5
Department of Genetics and Microbiology, Institute of Microbiology and Biotechnology, Maria Curie-Sklodowska University, Akademicka 19, 20-033 Lublin, Poland
*
Authors to whom correspondence should be addressed.
Int. J. Mol. Sci. 2021, 22(2), 485; https://doi.org/10.3390/ijms22020485
Submission received: 3 December 2020 / Revised: 3 January 2021 / Accepted: 4 January 2021 / Published: 6 January 2021
(This article belongs to the Special Issue Interactions of Cells with Biomaterials for Regenerative Medicine)
Browse Figures
Versions Notes

Abstract

Raman spectroscopic imaging and mapping were applied to characterise three-compound ceramic composite biomaterial consisting of chitosan, β-1,3-d-glucan (curdlan) and hydroxyapatite (HA) developed as a bone tissue engineering product (TEP). In this rapidly advancing domain of medical science, the urge for quick, reliable and specific method for products evaluation and tissue–implant interaction, in this case bone formation process, is constantly present. Two types of stem cells, adipose-derived stem cells (ADSCs) and bone marrow-derived stem cells (BMDSCs), were cultured on composite surface. Raman spectroscopic imaging provided advantageous information on molecular differences and spatial distribution of compounds within and between the cell-seeded and untreated samples at a microscopic level. With the use of this, it was possible to confirm composite biocompatibility and bioactivity in vitro. Deposition of HA and changes in its crystallinity along with protein adsorption proved new bone tissue formation in both mesenchymal stem cell samples, where the cells proliferated, differentiated and produced biomineralised extracellular matrix (ECM). The usefulness of spectroscopic Raman imaging was confirmed in tissue engineering in terms of both the organic and inorganic components considering composite–cells interaction.
Keywords: Raman spectroscopy; chemical imaging; hydroxyapatite composite; scaffold interface; tissue engineering Raman spectroscopy; chemical imaging; hydroxyapatite composite; scaffold interface; tissue engineering
Graphical Abstract

Share and Cite

MDPI and ACS Style

Kalisz, G.; Przekora, A.; Kazimierczak, P.; Gieroba, B.; Jedrek, M.; Grudzinski, W.; Gruszecki, W.I.; Ginalska, G.; Sroka-Bartnicka, A. Application of Raman Spectroscopic Imaging to Assess the Structural Changes at Cell-Scaffold Interface. Int. J. Mol. Sci. 2021, 22, 485. https://doi.org/10.3390/ijms22020485

AMA Style

Kalisz G, Przekora A, Kazimierczak P, Gieroba B, Jedrek M, Grudzinski W, Gruszecki WI, Ginalska G, Sroka-Bartnicka A. Application of Raman Spectroscopic Imaging to Assess the Structural Changes at Cell-Scaffold Interface. International Journal of Molecular Sciences. 2021; 22(2):485. https://doi.org/10.3390/ijms22020485

Chicago/Turabian Style

Kalisz, Grzegorz, Agata Przekora, Paulina Kazimierczak, Barbara Gieroba, Michal Jedrek, Wojciech Grudzinski, Wieslaw I. Gruszecki, Grazyna Ginalska, and Anna Sroka-Bartnicka. 2021. "Application of Raman Spectroscopic Imaging to Assess the Structural Changes at Cell-Scaffold Interface" International Journal of Molecular Sciences 22, no. 2: 485. https://doi.org/10.3390/ijms22020485

APA Style

Kalisz, G., Przekora, A., Kazimierczak, P., Gieroba, B., Jedrek, M., Grudzinski, W., Gruszecki, W. I., Ginalska, G., & Sroka-Bartnicka, A. (2021). Application of Raman Spectroscopic Imaging to Assess the Structural Changes at Cell-Scaffold Interface. International Journal of Molecular Sciences, 22(2), 485. https://doi.org/10.3390/ijms22020485

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop