Neuroprotective Effect of a Novel ATP-Synthase Inhibitor Bedaquiline in Cerebral Ischemia-Reperfusion Injury
Abstract
:1. Introduction
2. Results
2.1. Effect of BDQ Treatment on the Infarct Size and Neurological Impairment of Ischemic Rats
2.2. Effect of MCAO and BDQ Treatment on Mitochondrial Respiration
2.3. BDQ Effects on the Activity of ATPase
2.4. Effect of BDQ on Mitochondrial Calcium Retention Capacity
3. Discussion
4. Materials and Methods
4.1. Animals and Treatment
4.2. Transient Focal Ischemia Model
4.3. Neurological Evaluation
4.4. Evaluation of Infarct Size
4.5. Isolation of Mitochondria
4.6. Measurement of Mitochondrial Respiration
4.7. Measurement of Mitochondrial Calcium Retention Capacity
4.8. Measurement of the Enzymatic Activity of ATPase
4.9. Statistical Analysis
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Test | Neurological Deficit | Score |
---|---|---|
Forelimb extension (flexion) | Mild | 1 |
Twisting of the torso | Mild-moderate | 2 |
Circling to the right | Moderate | 3 |
Falling to the right | Severe | 4 |
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Umbrasas, D.; Arandarcikaite, O.; Grigaleviciute, R.; Stakauskas, R.; Borutaite, V. Neuroprotective Effect of a Novel ATP-Synthase Inhibitor Bedaquiline in Cerebral Ischemia-Reperfusion Injury. Int. J. Mol. Sci. 2021, 22, 9717. https://doi.org/10.3390/ijms22189717
Umbrasas D, Arandarcikaite O, Grigaleviciute R, Stakauskas R, Borutaite V. Neuroprotective Effect of a Novel ATP-Synthase Inhibitor Bedaquiline in Cerebral Ischemia-Reperfusion Injury. International Journal of Molecular Sciences. 2021; 22(18):9717. https://doi.org/10.3390/ijms22189717
Chicago/Turabian StyleUmbrasas, Danielius, Odeta Arandarcikaite, Ramune Grigaleviciute, Rimantas Stakauskas, and Vilmante Borutaite. 2021. "Neuroprotective Effect of a Novel ATP-Synthase Inhibitor Bedaquiline in Cerebral Ischemia-Reperfusion Injury" International Journal of Molecular Sciences 22, no. 18: 9717. https://doi.org/10.3390/ijms22189717