Coralyne Radiosensitizes A549 Cells by Upregulation of CDKN1A Expression to Attenuate Radiation Induced G2/M Block of the Cell Cycle
Abstract
:1. Introduction
2. Results
2.1. Kinetics of Coralyne Uptake by A549 Cells
2.2. Toxicity of Coralyne in A549 Cells
2.3. Coralyne Modulates Radiation-Induced Clonogenic Survival of A549 Cells
2.4. Coralyne Abrogates the G2/M Phase Arrest Caused by Ionizing Radiation in A549 Cells
2.5. Combined Treatment with Coralyne and Ionizing Radiation Up-Regulates the CDKN1A Gene Expression
2.6. Effect of Coralyne and IR on Protein Expression
2.7. Effects of Coralyne and IR on the Cytoskeleton Organization
3. Discussion
4. Materials and Methods
4.1. Cell Culture
4.2. Exposure of Cells to Coralyne and IR
4.3. Measurement of Coralyne Uptake by Flow Cytometry
4.4. Evaluation of Cellular Localization of Coralyne by Confocal Microscopy
4.5. Propidium Iodide (PI) Staining
4.6. Clonogenic Assay
4.7. Cell Cycle Analysis
4.8. RT-PCR Analysis
4.9. Western Blot Analysis
4.10. Analysis of Cytoskeletal Organization
4.11. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Węgierek-Ciuk, A.; Arabski, M.; Ciepluch, K.; Brzóska, K.; Lisowska, H.; Czerwińska, M.; Stępkowski, T.; Lis, K.; Lankoff, A. Coralyne Radiosensitizes A549 Cells by Upregulation of CDKN1A Expression to Attenuate Radiation Induced G2/M Block of the Cell Cycle. Int. J. Mol. Sci. 2021, 22, 5791. https://doi.org/10.3390/ijms22115791
Węgierek-Ciuk A, Arabski M, Ciepluch K, Brzóska K, Lisowska H, Czerwińska M, Stępkowski T, Lis K, Lankoff A. Coralyne Radiosensitizes A549 Cells by Upregulation of CDKN1A Expression to Attenuate Radiation Induced G2/M Block of the Cell Cycle. International Journal of Molecular Sciences. 2021; 22(11):5791. https://doi.org/10.3390/ijms22115791
Chicago/Turabian StyleWęgierek-Ciuk, Aneta, Michał Arabski, Karol Ciepluch, Kamil Brzóska, Halina Lisowska, Malwina Czerwińska, Tomasz Stępkowski, Krzysztof Lis, and Anna Lankoff. 2021. "Coralyne Radiosensitizes A549 Cells by Upregulation of CDKN1A Expression to Attenuate Radiation Induced G2/M Block of the Cell Cycle" International Journal of Molecular Sciences 22, no. 11: 5791. https://doi.org/10.3390/ijms22115791