Characterization of the TCR β Chain CDR3 Repertoire in Subarachnoid Hemorrhage Patients with Delayed Cerebral Ischemia
Abstract
:1. Introduction
2. Results
2.1. Clinical Characteristics of the Study
2.2. Sequence, Clonality, and CDR3 Length of TCRβ Chain CDR3 Repertoire
2.3. Distribution and Comparison of TRBV and TRBJ
3. Discussion
4. Materials and Methods
4.1. Study Population
4.2. DNA Extraction
4.3. High-Throughput Sequencing of the CDR3 Region
4.4. Analysis of the TCRβ Chain CDR3 Repertoire
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
Abbreviations
AMI | Acute myocardial infarction |
CDR | Complementary determining region |
DCI | Delayed cerebral ischemia |
PCR | Polymerase chain reaction |
SAH | Subarachnoid hemorrhage |
TCR | T cell receptor |
TCRB | TCR β chain |
References
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Pt No. | Age | Sex | Hunt and Hess Grade | Fisher Grade | Days of DCI | Aneurysm Site | Chemical Angioplasty | Underlying Medical Conditions |
---|---|---|---|---|---|---|---|---|
1 | 58 | F | III | 3 | 8 | BA | No | Hypertension |
2 | 60 | M | IV | 3 | 9 | A-com | No | Diabetes mellitus |
3 | 53 | F | III | 4 | 5 | P-com | No | Hypertension, hyperlipidemia |
4 | 52 | F | IV | 4 | 8 | MCA | Yes * | Hypertension, hyperlipidemia |
5 | 57 | F | IV | 4 | 7 | MCA | Yes * | Hypertension |
Pt | gDNA 1 | N.A 2 | Frame | Sequences (A.A.) 3 | ||||
---|---|---|---|---|---|---|---|---|
No. | Group | Amount (ng) | Total | Unique | Total | Unique | Clonality | Max Frequency (%) |
1 | Adm | 670.38 | 4590 | 3104 | 3747 | 2464 | 0.087 | 4.29 |
DCI | 670.40 | 5125 | 3497 | 4186 | 2785 | 0.045 | 2.01 | |
2 | Adm | 666.78 | 1614 | 1079 | 1270 | 860 | 0.089 | 5.27 |
DCI | 465.30 | 1879 | 1402 | 1527 | 1148 | 0.056 | 3.41 | |
3 | Adm | 668.64 | 7327 | 4623 | 5829 | 3652 | 0.124 | 7.92 |
DCI | 665.62 | 15670 | 10163 | 12555 | 8073 | 0.116 | 6.89 | |
4 | Adm | 668.07 | 5773 | 4918 | 4804 | 4120 | 0.017 | 0.97 |
DCI | 665.12 | 5387 | 4955 | 4498 | 4128 | 0.005 | 0.28 | |
5 | Adm | 717.79 | 7428 | 5650 | 6177 | 4576 | 0.053 | 1.95 |
DCI | 756.19 | 5072 | 4324 | 4238 | 3569 | 0.023 | 0.97 |
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Kim, B.J.; Youn, D.H.; Kim, Y.; Jeon, J.P. Characterization of the TCR β Chain CDR3 Repertoire in Subarachnoid Hemorrhage Patients with Delayed Cerebral Ischemia. Int. J. Mol. Sci. 2020, 21, 3149. https://doi.org/10.3390/ijms21093149
Kim BJ, Youn DH, Kim Y, Jeon JP. Characterization of the TCR β Chain CDR3 Repertoire in Subarachnoid Hemorrhage Patients with Delayed Cerebral Ischemia. International Journal of Molecular Sciences. 2020; 21(9):3149. https://doi.org/10.3390/ijms21093149
Chicago/Turabian StyleKim, Bong Jun, Dong Hyuk Youn, Youngmi Kim, and Jin Pyeong Jeon. 2020. "Characterization of the TCR β Chain CDR3 Repertoire in Subarachnoid Hemorrhage Patients with Delayed Cerebral Ischemia" International Journal of Molecular Sciences 21, no. 9: 3149. https://doi.org/10.3390/ijms21093149