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Open AccessArticle

Major Latex Protein MdMLP423 Negatively Regulates Defense against Fungal Infections in Apple

by 1,2,†, 1,2,†, 1,2, 1,2, 1,2, 1,2 and 1,2,*
1
Research Institute of Pomology, Chinese Academy of Agricultural Sciences, Xingcheng 125100, China
2
Key Laboratory of Biology and Genetic Improvement of Horticultural Crops, Xingcheng 125100, China
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Int. J. Mol. Sci. 2020, 21(5), 1879; https://doi.org/10.3390/ijms21051879
Received: 3 January 2020 / Revised: 9 March 2020 / Accepted: 9 March 2020 / Published: 10 March 2020
(This article belongs to the Special Issue Plant Disease Resistance)
Major latex proteins (MLPs) play critical roles in plants defense and stress responses. However, the roles of MLPs from apple (Malus × domestica) have not been clearly identified. In this study, we focused on the biological role of MdMLP423, which had been previously characterized as a potential pathogenesis-related gene. Phylogenetic analysis and conserved domain analysis indicated that MdMLP423 is a protein with a ‘Gly-rich loop’ (GXGGXG) domain belonging to the Bet v_1 subfamily. Gene expression profiles showed that MdMLP423 is mainly expressed in flowers. In addition, the expression of MdMLP423 was significantly inhibited by Botryosphaeria berengeriana f. sp. piricola (BB) and Alternaria alternata apple pathotype (AAAP) infections. Apple calli overexpressing MdMLP423 had lower expression of resistance-related genes, and were more sensitive to infection with BB and AAAP compared with non-transgenic calli. RNA-seq analysis of MdMLP423-overexpressing calli and non-transgenic calli indicated that MdMLP423 regulated the expression of a number of differentially expressed genes (DEGs) and transcription factors, including genes involved in phytohormone signaling pathways, cell wall reinforcement, and genes encoding the defense-related proteins, AP2-EREBP, WRKY, MYB, NAC, Zinc finger protein, and ABI3. Taken together, our results demonstrate that MdMLP423 negatively regulates apple resistance to BB and AAAP infections by inhibiting the expression of defense- and stress-related genes and transcription factors. View Full-Text
Keywords: major latex proteins; fungal infections; apple (Malus × domestica); defense and stress responses major latex proteins; fungal infections; apple (Malus × domestica); defense and stress responses
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MDPI and ACS Style

He, S.; Yuan, G.; Bian, S.; Han, X.; Liu, K.; Cong, P.; Zhang, C. Major Latex Protein MdMLP423 Negatively Regulates Defense against Fungal Infections in Apple. Int. J. Mol. Sci. 2020, 21, 1879. https://doi.org/10.3390/ijms21051879

AMA Style

He S, Yuan G, Bian S, Han X, Liu K, Cong P, Zhang C. Major Latex Protein MdMLP423 Negatively Regulates Defense against Fungal Infections in Apple. International Journal of Molecular Sciences. 2020; 21(5):1879. https://doi.org/10.3390/ijms21051879

Chicago/Turabian Style

He, Shanshan; Yuan, Gaopeng; Bian, Shuxun; Han, Xiaolei; Liu, Kai; Cong, Peihua; Zhang, Caixia. 2020. "Major Latex Protein MdMLP423 Negatively Regulates Defense against Fungal Infections in Apple" Int. J. Mol. Sci. 21, no. 5: 1879. https://doi.org/10.3390/ijms21051879

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