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The Relationships Between Stress, Mental Disorders, and Epigenetic Regulation of BDNF
Article

BDNF Expression in Cortical GABAergic Interneurons

1
Institute of Integrative Neuroanatomy, Charité-Universitätsmedizin Berlin, Campus Mitte, 10117 Berlin, Germany
2
Bernstein Center for Computational Neuroscience (BCCN) Berlin, 10115 Berlin, Germany
3
Institute of Neurophysiology, Charité - Universitätsmedizin Berlin, Campus Mitte, 10117 Berlin, Germany
4
Departments of Genetic and Behavioral Neuroscience, Graduate School of Medicine, Gunma University, Graduate School of Medicine, Maebashi City 371-8511, Japan
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(5), 1567; https://doi.org/10.3390/ijms21051567
Received: 8 January 2020 / Revised: 7 February 2020 / Accepted: 21 February 2020 / Published: 25 February 2020
(This article belongs to the Special Issue Brain-Derived Neurotrophic Factor 2020)
Brain-derived neurotrophic factor (BDNF) is a major neuronal growth factor that is widely expressed in the central nervous system. It is synthesized as a glycosylated precursor protein, (pro)BDNF and post-translationally converted to the mature form, (m)BDNF. BDNF is known to be produced and secreted by cortical glutamatergic principal cells (PCs); however, it remains a question whether it can also be synthesized by other neuron types, in particular, GABAergic interneurons (INs). Therefore, we utilized immunocytochemical labeling and reverse transcription quantitative PCR (RT-qPCR) to investigate the cellular distribution of proBDNF and its RNA in glutamatergic and GABAergic neurons of the mouse cortex. Immunofluorescence labeling revealed that mBDNF, as well as proBDNF, localized to both the neuronal populations in the hippocampus. The precursor proBDNF protein showed a perinuclear distribution pattern, overlapping with the rough endoplasmic reticulum (ER), the site of protein synthesis. RT-qPCR of samples obtained using laser capture microdissection (LCM) or fluorescence-activated cell sorting (FACS) of hippocampal and cortical neurons further demonstrated the abundance of BDNF transcripts in both glutamatergic and GABAergic cells. Thus, our data provide compelling evidence that BDNF can be synthesized by both principal cells and INs of the cortex. View Full-Text
Keywords: BDNF; hippocampus; neocortex; GABAergic interneurons; laser capture microdissection; RT-qPCR; FACS BDNF; hippocampus; neocortex; GABAergic interneurons; laser capture microdissection; RT-qPCR; FACS
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MDPI and ACS Style

Tomás, F.J.B.; Turko, P.; Heilmann, H.; Trimbuch, T.; Yanagawa, Y.; Vida, I.; Münster-Wandowski, A. BDNF Expression in Cortical GABAergic Interneurons. Int. J. Mol. Sci. 2020, 21, 1567. https://doi.org/10.3390/ijms21051567

AMA Style

Tomás FJB, Turko P, Heilmann H, Trimbuch T, Yanagawa Y, Vida I, Münster-Wandowski A. BDNF Expression in Cortical GABAergic Interneurons. International Journal of Molecular Sciences. 2020; 21(5):1567. https://doi.org/10.3390/ijms21051567

Chicago/Turabian Style

Tomás, Federico J.B., Paul Turko, Heike Heilmann, Thorsten Trimbuch, Yuchio Yanagawa, Imre Vida, and Agnieszka Münster-Wandowski. 2020. "BDNF Expression in Cortical GABAergic Interneurons" International Journal of Molecular Sciences 21, no. 5: 1567. https://doi.org/10.3390/ijms21051567

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