Aquaporin 1, 3, and 5 Patterns in Salivary Gland Mucoepidermoid Carcinoma: Expression in Surgical Specimens and an In Vitro Pilot Study

Stamboni, Mérin Barbara; Gomes, Ágatha Nagli de Mello; Souza, Milena Monteiro de; Oliveira, Katia Klug; Arruda, Claudia Fabiana Joca; de Paula, Fernanda; Bettim, Barbara Beltrame; Marques, Márcia Martins; Kowalski, Luiz Paulo; Pinto, Clóvis Antônio Lopes; Arana-Chavez, Victor Elias; Lourenço, Silvia Vanessa; Coutinho-Camillo, Cláudia Malheiros

doi:10.3390/ijms21041287

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Aquaporin 1, 3, and 5 Patterns in Salivary Gland Mucoepidermoid Carcinoma: Expression in Surgical Specimens and an In Vitro Pilot Study

Mérin Barbara Stamboni

^1,2,

Ágatha Nagli de Mello Gomes

¹,

Milena Monteiro de Souza

Katia Klug Oliveira

¹,

Claudia Fabiana Joca Arruda

Fernanda de Paula

Barbara Beltrame Bettim

¹,

Márcia Martins Marques

^4,†,

Luiz Paulo Kowalski

⁵,

Clóvis Antônio Lopes Pinto

⁶,

Victor Elias Arana-Chavez

⁷

Silvia Vanessa Lourenço

and

Cláudia Malheiros Coutinho-Camillo

^1,2,*

International Research Center, A.C.Camargo Cancer Center, Rua Taguá 440, São Paulo 01508-010, Brazil

National Institute of Science and Technology in Oncogenomics and Therapeutic Innovation, São Paulo 05403-010, SP, Brazil

Department of General Pathology, Dental School, University of São Paulo, São Paulo 05508-000, Brazil

⁴

Department of Restorative Dentistry, School of Dentistry, University of São Paulo, São Paulo 05508-000, Brazil

⁵

Department of Head and Neck Surgery and Otorhinolaryngology, A.C.Camargo Cancer Center, São Paulo 01525-001, Brazil

⁶

Department of Anatomic Pathology, A.C.Camargo Cancer Center, São Paulo 01525-001, Brazil

⁷

Laboratory of Oral Biology, Department of Biomaterials and Oral Biology, Dental School, University of São Paulo, São Paulo 05508-000, Brazil

Author to whom correspondence should be addressed.

^†

Current address: Post-Graduation Program, School of Dentistry, Ibirapuera University, São Paulo 04661-100, Brazil.

Int. J. Mol. Sci. 2020, 21(4), 1287; https://doi.org/10.3390/ijms21041287

Received: 27 December 2019 / Revised: 16 January 2020 / Accepted: 22 January 2020 / Published: 14 February 2020

(This article belongs to the Special Issue Aquaporins: Water Channels Essential for Living Organisms 3.0)

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Abstract

Salivary gland aquaporins (AQPs) are essential for the control of saliva production and maintenance of glandular structure. However, little is known of their role in salivary gland neoplasia. Salivary gland tumors comprise a heterogeneous group of lesions, featuring variable histological characteristics and diverse clinical behaviors. Mucoepidermoid carcinoma (MEC) is the most common salivary gland malignancy. The aim of this study was to evaluate the expression of AQP1, AQP3, and AQP5 in 24 MEC samples by immunohistochemistry. AQP1 expression was observed in vascular endothelium throughout the tumor stroma. AQP3 was expressed in epidermoid and mucosal cells and AQP5 was expressed in mucosal cells of MEC. These proteins were expressed in the human MEC cell line UH-HMC-3A. Cellular ultrastructural aspects were analyzed by electron microscopy to certificate the tumor cell phenotype. In summary, our results show that, despite the fact that these molecules are important for salivary gland physiology, they may not play a distinct role in tumorigenesis in MEC. Additionally, the in vitro model may offer new possibilities to further investigate mechanisms of these molecules in tumor biology and their real significance in prognosis and possible target therapies. View Full-Text

Keywords: aquaporins; mucoepidermoid carcinoma; immunohistochemistry; immunofluorescence; electron microscopy

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MDPI and ACS Style

Stamboni, M.B.; Gomes, Á.N.d.M.; Souza, M.M.d.; Oliveira, K.K.; Arruda, C.F.J.; de Paula, F.; Bettim, B.B.; Marques, M.M.; Kowalski, L.P.; Pinto, C.A.L.; Arana-Chavez, V.E.; Lourenço, S.V.; Coutinho-Camillo, C.M. Aquaporin 1, 3, and 5 Patterns in Salivary Gland Mucoepidermoid Carcinoma: Expression in Surgical Specimens and an In Vitro Pilot Study. Int. J. Mol. Sci. 2020, 21, 1287. https://doi.org/10.3390/ijms21041287

AMA Style

Stamboni MB, Gomes ÁNdM, Souza MMd, Oliveira KK, Arruda CFJ, de Paula F, Bettim BB, Marques MM, Kowalski LP, Pinto CAL, Arana-Chavez VE, Lourenço SV, Coutinho-Camillo CM. Aquaporin 1, 3, and 5 Patterns in Salivary Gland Mucoepidermoid Carcinoma: Expression in Surgical Specimens and an In Vitro Pilot Study. International Journal of Molecular Sciences. 2020; 21(4):1287. https://doi.org/10.3390/ijms21041287

Chicago/Turabian Style

Stamboni, Mérin Barbara, Ágatha Nagli de Mello Gomes, Milena Monteiro de Souza, Katia Klug Oliveira, Claudia Fabiana Joca Arruda, Fernanda de Paula, Barbara Beltrame Bettim, Márcia Martins Marques, Luiz Paulo Kowalski, Clóvis Antônio Lopes Pinto, Victor Elias Arana-Chavez, Silvia Vanessa Lourenço, and Cláudia Malheiros Coutinho-Camillo. 2020. "Aquaporin 1, 3, and 5 Patterns in Salivary Gland Mucoepidermoid Carcinoma: Expression in Surgical Specimens and an In Vitro Pilot Study" International Journal of Molecular Sciences 21, no. 4: 1287. https://doi.org/10.3390/ijms21041287

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Aquaporin 1, 3, and 5 Patterns in Salivary Gland Mucoepidermoid Carcinoma: Expression in Surgical Specimens and an In Vitro Pilot Study

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