Comparative Transcriptome Analysis Provides Insights into the Polyunsaturated Fatty Acid Synthesis Regulation of Fat-1 Transgenic Sheep
Abstract
:1. Introduction
2. Results
2.1. Generation of Fat-1 Transgenic Fetuses and Lambs
2.2. Global Gene Expression Profiles of Different Tissue and Skin Cell Samples
2.3. Gene Expression Differences between Abnormal and Normal Fetuses
2.4. Gene Expression Differences between Fat-1 Transgenic-Silencing and -Expressing Skin Cells
2.5. Gene Expression Differences between Fat-1 Transgenic Fetuses and Postnatal Lambs
2.6. PUFA Contents between 100-day Fat-1 Transgenic Fetuses and Wild Type Fetuses
3. Discussion
4. Materials and Methods
4.1. Ethical Statement
4.2. Generation of Fat-1 Transgenic Lambs and Sample Collection for RNA-seq
4.3. RNA Isolation, Library Construction, and Transcriptome Sequencing
4.4. Read Mapping and Analysis of Differential Gene Expression
4.5. KEGG Pathway Enrichment and PPI Network Analysis
4.6. Quantitative Real-Time PCR (qPCR) Validation
4.7. Determination of Polyunsaturated Fatty Acids
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Data Availability
Conflicts of Interest
Abbreviations
DAF_1 | Abnormal developed fetus at 100 gestational days |
DAF_2 | normal fetus at 100 gestational days |
DAF_4 | postnatal lambs |
DAFG5 | Fat-1 transgenic-silencing skin cells |
DAFG6 | Fat-1 transgenic-expressing skin cells |
PUFAs | Polyunsaturated fatty acids |
GMO | Genetically modified organism |
DEG | Differentially expressed gene |
FPKM | Fragments per kilobase of exon per million mapped reads |
KEGG | Kyoto Encyclopedia of Genes and Genomes |
PCA | Principal components analysis |
PPI | Protein and protein interaction network |
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Luo, R.; Zheng, Z.; Yang, C.; Zhang, X.; Cheng, L.; Su, G.; Bai, C.; Li, G. Comparative Transcriptome Analysis Provides Insights into the Polyunsaturated Fatty Acid Synthesis Regulation of Fat-1 Transgenic Sheep. Int. J. Mol. Sci. 2020, 21, 1121. https://doi.org/10.3390/ijms21031121
Luo R, Zheng Z, Yang C, Zhang X, Cheng L, Su G, Bai C, Li G. Comparative Transcriptome Analysis Provides Insights into the Polyunsaturated Fatty Acid Synthesis Regulation of Fat-1 Transgenic Sheep. International Journal of Molecular Sciences. 2020; 21(3):1121. https://doi.org/10.3390/ijms21031121
Chicago/Turabian StyleLuo, Rongsong, Zhong Zheng, Chunrong Yang, Xiaoran Zhang, Lei Cheng, Guanghua Su, Chunling Bai, and Guangpeng Li. 2020. "Comparative Transcriptome Analysis Provides Insights into the Polyunsaturated Fatty Acid Synthesis Regulation of Fat-1 Transgenic Sheep" International Journal of Molecular Sciences 21, no. 3: 1121. https://doi.org/10.3390/ijms21031121