Next Article in Journal
Relationship of 2D Affinity to T Cell Functional Outcomes
Next Article in Special Issue
Comparative Transcriptome Analysis of Rutabaga (Brassica napus) Cultivars Indicates Activation of Salicylic Acid and Ethylene-Mediated Defenses in Response to Plasmodiophora brassicae
Previous Article in Journal
The Ups and Downs of Metabolism during the Lifespan of a T Cell
Previous Article in Special Issue
Epigenetic and Metabolic Changes in Root-Knot Nematode-Plant Interactions
Open AccessArticle

A Comprehensive Analysis of MicroRNAs Expressed in Susceptible and Resistant Rice Cultivars during Rhizoctonia solani AG1-IA Infection Causing Sheath Blight Disease

1
ICAR-Indian Institute of Rice Research, Hyderabad 500030, India
2
Bioinformatics Lab, ICAR-National Academy of Agricultural Research Management, Hyderabad 500030, India
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Int. J. Mol. Sci. 2020, 21(21), 7974; https://doi.org/10.3390/ijms21217974
Received: 10 September 2020 / Revised: 8 October 2020 / Accepted: 8 October 2020 / Published: 27 October 2020
(This article belongs to the Special Issue Plant Disease Resistance)
MicroRNAs regulate plant responses to fungal infections and immunity. In this study, miRNAs were identified in six rice cultivars during a Rhizoctonia solani Kühn AG1-IA infection using a deep sequencing approach. Known and novel miRNAs were analyzed in these rice cultivars, and a set of fungal infection/immunity-associated miRNAs and target genes were quantified by reverse transcription (RT)-qPCR in six rice cultivars. Additionally, the relative expression of these miRNAs was analyzed in different time points of the infection, wild species of rice, and in response to different strains of R. solani. Osa-miR1320-5p showed preferential expression during the fungal infection in all the six rice genotypes, while Osa-miR156d, Osa-miR159b, Osa-miR820c, and Osa-miR1876 were differentially regulated in susceptible and resistant genotypes. A greater degree of downregulation of miRNAs was observed during the initial time points of infection (24–72 h), suggesting a maximum molecular activity of rice-R. solani interaction and resistance response of the host during the early phase of infection. After R. solani infection, the expression of Osa-miR820c and Osa-miR156d was downregulated in Oryza rufipogon, O. alta, O. latifolia, and O. minuta, while Osa-miR397b was downregulated in all the wild rice species except O. officinalis. This study provided comprehensive information on the repertoire of miRNAs expressed in six sheath blight disease-susceptible and resistant indica and aus rice cultivars. View Full-Text
Keywords: fungi; Oryza sativa; miRNA; strain; wild rice; gene expression fungi; Oryza sativa; miRNA; strain; wild rice; gene expression
Show Figures

Figure 1

MDPI and ACS Style

Chopperla, R.; Mangrauthia, S.K.; Bhaskar Rao, T.; Balakrishnan, M.; Balachandran, S.M.; Prakasam, V.; Channappa, G. A Comprehensive Analysis of MicroRNAs Expressed in Susceptible and Resistant Rice Cultivars during Rhizoctonia solani AG1-IA Infection Causing Sheath Blight Disease. Int. J. Mol. Sci. 2020, 21, 7974. https://doi.org/10.3390/ijms21217974

AMA Style

Chopperla R, Mangrauthia SK, Bhaskar Rao T, Balakrishnan M, Balachandran SM, Prakasam V, Channappa G. A Comprehensive Analysis of MicroRNAs Expressed in Susceptible and Resistant Rice Cultivars during Rhizoctonia solani AG1-IA Infection Causing Sheath Blight Disease. International Journal of Molecular Sciences. 2020; 21(21):7974. https://doi.org/10.3390/ijms21217974

Chicago/Turabian Style

Chopperla, Ramakrishna; Mangrauthia, Satendra K.; Bhaskar Rao, Talluri; Balakrishnan, Marudamuthu; Balachandran, Sena M.; Prakasam, Vellaisamy; Channappa, Gireesh. 2020. "A Comprehensive Analysis of MicroRNAs Expressed in Susceptible and Resistant Rice Cultivars during Rhizoctonia solani AG1-IA Infection Causing Sheath Blight Disease" Int. J. Mol. Sci. 21, no. 21: 7974. https://doi.org/10.3390/ijms21217974

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop