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Open AccessArticle

Aquaglyceroporins Are Differentially Expressed in Beige and White Adipocytes

1
Research Institute for Medicines (iMed.ULisboa), Faculty of Pharmacy, Universidade de Lisboa, 1649-003 Lisboa, Portugal
2
Department of Biochemistry and Human Biology, Faculty of Pharmacy, Universidade de Lisboa, 1649-003 Lisboa, Portugal
3
Department of Biochemistry and Molecular Biomedicine, Faculty of Biology, Institute of Biomedicine of the University of Barcelona, 08028 Barcelona, Spain
4
CIBER de Diabetes y Enfermedades Metabólicas Asociadas, Instituto de Salud Carlos III, 8029 Madrid, Spain
5
Institute for Research in Biomedicine (IRB Barcelona), 08028 Barcelona, Spain
*
Authors to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(2), 610; https://doi.org/10.3390/ijms21020610
Received: 29 December 2019 / Revised: 13 January 2020 / Accepted: 15 January 2020 / Published: 17 January 2020
(This article belongs to the Special Issue Channels and Transporters in Cells and Tissues)
Browning of white adipocytes has been proposed as a powerful strategy to overcome metabolic complications, since brown adipocytes are more catabolic, expending energy as a heat form. However, the biological pathways involved in the browning process are still unclear. Aquaglyceroporins are a sub-class of aquaporin water channels that also permeate glycerol and are involved in body energy homeostasis. In the adipose tissue, aquaporin-7 (AQP7) is the most representative isoform, being crucial for white adipocyte fully differentiation and glycerol metabolism. The altered expression of AQP7 is involved in the onset of obesity and metabolic disorders. Herein, we investigated if aquaglyceroporins are implicated in beige adipocyte differentiation, similar to white cells. Thus, we optimized a protocol of murine 3T3-L1 preadipocytes browning that displayed increased beige and decreased white adipose tissue features at both gene and protein levels and evaluated aquaporin expression patterns along the differentiation process together with cellular lipid content. Our results revealed that AQP7 and aquaporin-9 (AQP9) expression was downregulated throughout beige adipocyte differentiation compared to white differentiation, which may be related to the beige physiological role of heat production from oxidative metabolism, contrasting with the anabolic/catabolic lipid metabolism requiring glycerol gateways occurring in white adipose cells. View Full-Text
Keywords: aquaporin; adipocytes; browning; obesity; metabolic diseases aquaporin; adipocytes; browning; obesity; metabolic diseases
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MDPI and ACS Style

da Silva, I.V.; Díaz-Sáez, F.; Zorzano, A.; Gumà, A.; Camps, M.; Soveral, G. Aquaglyceroporins Are Differentially Expressed in Beige and White Adipocytes. Int. J. Mol. Sci. 2020, 21, 610. https://doi.org/10.3390/ijms21020610

AMA Style

da Silva IV, Díaz-Sáez F, Zorzano A, Gumà A, Camps M, Soveral G. Aquaglyceroporins Are Differentially Expressed in Beige and White Adipocytes. International Journal of Molecular Sciences. 2020; 21(2):610. https://doi.org/10.3390/ijms21020610

Chicago/Turabian Style

da Silva, Inês V.; Díaz-Sáez, Francisco; Zorzano, António; Gumà, Anna; Camps, Marta; Soveral, Graça. 2020. "Aquaglyceroporins Are Differentially Expressed in Beige and White Adipocytes" Int. J. Mol. Sci. 21, no. 2: 610. https://doi.org/10.3390/ijms21020610

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