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Open AccessArticle

Release of Cholecystokinin from Rat Intestinal Mucosal Cells and the Enteroendocrine Cell Line STC-1 in Response to Maleic and Succinic Acid, Fermentation Products of Alcoholic Beverages

1
University Hospital Schleswig-Holstein, Campus Kiel, 24105 Kiel, Germany
2
Research Unit of Biomedicine, University of Oulu, 90014 Oulu, Finland
3
Krankenhaus St. Joseph-Stift, 28209 Bremen, Germany
4
Department of Gastroenterology and Metabolism, Poznan University of Medical Sciences, 61-701 Poznan, Poland
5
Medical Research Center Oulu and Oulu University Hospital, 90014 Oulu, Finland
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(2), 589; https://doi.org/10.3390/ijms21020589
Received: 18 November 2019 / Revised: 8 January 2020 / Accepted: 14 January 2020 / Published: 16 January 2020
(This article belongs to the Special Issue Gut Hormone: From Molecular Mechanism to Clinical Aspects)
Alcoholic beverages stimulate pancreatic enzyme secretions by inducing cholecystokinin (CCK) release. CCK is the major stimulatory hormone of pancreatic exocrine secretions, secreted from enteroendocrine I-cells of the intestine. Fermentation products of alcoholic beverages, such as maleic and succinic acids, influence gastric acid secretions. We hypothesize that maleic and succinic acids stimulate pancreatic exocrine secretions during beer and wine ingestion by increasing CCK secretions. Therefore, the effects of maleic and succinic acids on CCK release were studied in duodenal mucosal cells and the enteroendocrine cell line STC-1. Mucosal cells were perfused for 30 min with 5 min sampling intervals, STC-1 cells were studied under static incubation for 15 min, and supernatants were collected for CCK measurements. Succinate and maleate-induced CCK release were investigated. Succinate and maleate doses dependently stimulated CCK secretions from mucosal cells and STC-1 cells. Diltiazem, a calcium channel blocker, significantly inhibited succinate and maleate-induced CCK secretions from mucosal cells and STC-1 cells. Maleate and succinate did not show cytotoxicity in STC-1 cells. Our results indicate that succinate and maleate are novel CCK-releasing factors in fermented alcoholic beverages and could contribute to pancreatic exocrine secretions and their pathophysiology. View Full-Text
Keywords: cholecystokinin (CCK); enteroendocrine cells STC-1; pancreatic secretions; pancreatitis; alcohol; fermentation; maleic and succinic acids; intracellular calcium cholecystokinin (CCK); enteroendocrine cells STC-1; pancreatic secretions; pancreatitis; alcohol; fermentation; maleic and succinic acids; intracellular calcium
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Egberts, J.-H.; Raza, G.S.; Wilgus, C.; Teyssen, S.; Kiehne, K.; Herzig, K.-H. Release of Cholecystokinin from Rat Intestinal Mucosal Cells and the Enteroendocrine Cell Line STC-1 in Response to Maleic and Succinic Acid, Fermentation Products of Alcoholic Beverages. Int. J. Mol. Sci. 2020, 21, 589.

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