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Article

Quantification of Iron Release from Native Ferritin and Magnetoferritin Induced by Vitamins B2 and C

1
Biomedical Center Martin, Jessenius Faculty of Medicine in Martin, Comenius University in Bratislava, Mala Hora 4, 036 01 Martin, Slovakia
2
Institute of Hydrology, Slovak Academy of Sciences, Dubravska cesta 9, 841 04 Bratislava, Slovakia
3
Department of Medical Biochemistry, Jessenius Faculty of Medicine, Comenius University in Bratislava, Mala Hora 4, 036 01 Martin, Slovakia
4
Institute of Experimental Physics, Slovak Academy of Sciences, Watsonova 47, 040 01 Kosice, Slovakia
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(17), 6332; https://doi.org/10.3390/ijms21176332
Received: 9 July 2020 / Revised: 23 August 2020 / Accepted: 30 August 2020 / Published: 31 August 2020
Various pathological processes in humans are associated with biogenic iron accumulation and the mineralization of iron oxide nanoparticles, especially magnetite. Ferritin has been proposed as a precursor to pathological magnetite mineralization. This study quantifies spectroscopically the release of ferrous ions from native ferritin and magnetoferritin as a model system for pathological ferritin in the presence of potent natural reducing agents (vitamins C and B2) over time. Ferrous cations are required for the transformation of ferrihydrite (physiological) into a magnetite (pathological) mineral core and are considered toxic at elevated levels. The study shows a significant difference in the reduction and iron release from native ferritin compared to magnetoferritin for both vitamins. The amount of reduced iron formed from a magnetoferritin mineral core is two to five times higher than from native ferritin. Surprisingly, increasing the concentration of the reducing agent affects only iron release from native ferritin. Magnetoferritin cores with different loading factors seem to be insensitive to different concentrations of vitamins. An alternative hypothesis of human tissue magnetite mineralization and the process of iron-induced pathology is proposed. The results could contribute to evidence of the molecular mechanisms of various iron-related pathologies, including neurodegeneration. View Full-Text
Keywords: ferritin; magnetoferritin; mineral core; reduction; ascorbic acid; riboflavin; ferrous ions; time dependence; toxicity ferritin; magnetoferritin; mineral core; reduction; ascorbic acid; riboflavin; ferrous ions; time dependence; toxicity
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MDPI and ACS Style

Strbak, O.; Balejcikova, L.; Kmetova, M.; Gombos, J.; Trancikova, A.; Pokusa, M.; Kopcansky, P. Quantification of Iron Release from Native Ferritin and Magnetoferritin Induced by Vitamins B2 and C. Int. J. Mol. Sci. 2020, 21, 6332. https://doi.org/10.3390/ijms21176332

AMA Style

Strbak O, Balejcikova L, Kmetova M, Gombos J, Trancikova A, Pokusa M, Kopcansky P. Quantification of Iron Release from Native Ferritin and Magnetoferritin Induced by Vitamins B2 and C. International Journal of Molecular Sciences. 2020; 21(17):6332. https://doi.org/10.3390/ijms21176332

Chicago/Turabian Style

Strbak, Oliver, Lucia Balejcikova, Martina Kmetova, Jan Gombos, Alzbeta Trancikova, Michal Pokusa, and Peter Kopcansky. 2020. "Quantification of Iron Release from Native Ferritin and Magnetoferritin Induced by Vitamins B2 and C" International Journal of Molecular Sciences 21, no. 17: 6332. https://doi.org/10.3390/ijms21176332

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