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Article

Long-Term Impact of Chemical and Alternative Fungicides Applied to Grapevine cv Nebbiolo on Berry Transcriptome

1
National Research Council, Institute for Sustainable Plant Protection, 10125 Turin, Italy
2
Department of Life Science and Systems Biology, Turin University, 10125 Turin, Italy
*
Author to whom correspondence should be addressed.
Current address: Department of Biology and Biotechnology “C. Darwin”, Sapienza University, 00185 Rome, Italy.
Int. J. Mol. Sci. 2020, 21(17), 6067; https://doi.org/10.3390/ijms21176067
Received: 25 July 2020 / Revised: 11 August 2020 / Accepted: 20 August 2020 / Published: 23 August 2020
(This article belongs to the Special Issue Cell-Specificity in Plants)
Viticulture is one of the horticultural systems in which antifungal treatments can be extremely frequent, with substantial economic and environmental costs. New products, such as biofungicides, resistance inducers and biostimulants, may represent alternative crop protection strategies respectful of the environmental sustainability and food safety. Here, the main purpose was to evaluate the systemic molecular modifications induced by biocontrol products as laminarin, resistance inducers (i.e., fosetyl-Al and potassium phosphonate), electrolyzed water and a standard chemical fungicide (i.e., metiram), on the transcriptomic profile of ‘Nebbiolo’ grape berries at harvest. In addition to a validation of the sequencing data through real-time polymerase chain reaction (PCR), for the first-time the expression of some candidate genes in different cell-types of berry skin (i.e., epidermal and hypodermal layers) was evaluated using the laser microdissection approach. Results showed that several considered antifungal treatments do not strongly affect the berry transcriptome profile at the end of season. Although some treatments do not activate long lasting molecular defense priming features in berry, some compounds appear to be more active in long-term responses. In addition, genes differentially expressed in the two-cell type populations forming the berry skin were found, suggesting a different function for the two-cell type populations. View Full-Text
Keywords: viticulture; berry transcriptome; laser microdissection viticulture; berry transcriptome; laser microdissection
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MDPI and ACS Style

Balestrini, R.; Ghignone, S.; Quiroga, G.; Fiorilli, V.; Romano, I.; Gambino, G. Long-Term Impact of Chemical and Alternative Fungicides Applied to Grapevine cv Nebbiolo on Berry Transcriptome. Int. J. Mol. Sci. 2020, 21, 6067. https://doi.org/10.3390/ijms21176067

AMA Style

Balestrini R, Ghignone S, Quiroga G, Fiorilli V, Romano I, Gambino G. Long-Term Impact of Chemical and Alternative Fungicides Applied to Grapevine cv Nebbiolo on Berry Transcriptome. International Journal of Molecular Sciences. 2020; 21(17):6067. https://doi.org/10.3390/ijms21176067

Chicago/Turabian Style

Balestrini, Raffaella, Stefano Ghignone, Gabriela Quiroga, Valentina Fiorilli, Irene Romano, and Giorgio Gambino. 2020. "Long-Term Impact of Chemical and Alternative Fungicides Applied to Grapevine cv Nebbiolo on Berry Transcriptome" International Journal of Molecular Sciences 21, no. 17: 6067. https://doi.org/10.3390/ijms21176067

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