Hydroxytyrosol Inhibits Protein Oligomerization and Amyloid Aggregation in Human Insulin
Abstract
:1. Introduction
2. Results
2.1. Inhibitory Effect of HT on Insulin Amyloid Aggregation
2.2. Effect of HT on Insulin Amyloid Toxicity
2.3. Effect of HT on Insulin Oligomerization State
2.4. HT-Insulin Interaction
3. Discussion
4. Materials and Methods
4.1. Materials
4.2. Insulin Preparation and Amyloid Aggregation
4.3. Circular Dichroism Measurements
4.4. Fluorescence Measurements
4.5. Dynamic Light Scattering
4.6. Cell Cultures and Treatments
4.7. MTT Assay
4.8. Statistical Analysis
4.9. Data Availability
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Sirangelo, I.; Borriello, M.; Vilasi, S.; Iannuzzi, C. Hydroxytyrosol Inhibits Protein Oligomerization and Amyloid Aggregation in Human Insulin. Int. J. Mol. Sci. 2020, 21, 4636. https://doi.org/10.3390/ijms21134636
Sirangelo I, Borriello M, Vilasi S, Iannuzzi C. Hydroxytyrosol Inhibits Protein Oligomerization and Amyloid Aggregation in Human Insulin. International Journal of Molecular Sciences. 2020; 21(13):4636. https://doi.org/10.3390/ijms21134636
Chicago/Turabian StyleSirangelo, Ivana, Margherita Borriello, Silvia Vilasi, and Clara Iannuzzi. 2020. "Hydroxytyrosol Inhibits Protein Oligomerization and Amyloid Aggregation in Human Insulin" International Journal of Molecular Sciences 21, no. 13: 4636. https://doi.org/10.3390/ijms21134636