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Article

Screening for Cholesterol-Lowering Probiotics from Lactic Acid Bacteria Isolated from Corn Silage Based on Three Hypothesized Pathways

1
Institute of Food Science and Technology, Chinese Academy of Agricultural Science, Beijing 100193, China
2
Department of Food and Bio-engineering, Beijing Vocational College of Agriculture, Beijing 102442, China
3
Laboratory of Environment and Livestock Products, Henan University of Science and Technology, Luoyang 471023, China
4
Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology & Business University, Beijing 100048, China
*
Authors to whom correspondence should be addressed.
Int. J. Mol. Sci. 2019, 20(9), 2073; https://doi.org/10.3390/ijms20092073
Received: 28 March 2019 / Revised: 11 April 2019 / Accepted: 24 April 2019 / Published: 26 April 2019
(This article belongs to the Section Bioactives and Nutraceuticals)
A total of 85 strains of lactic acid bacteria were isolated from corn silage in this study and analyzed in vitro for their cholesterol removal, NPC1L1 protein down-regulation and bile salt deconjugation ability, respectively. Nineteen strains were selected for further analysis for their probiotic potential. Finally, 3 strains showing better probiotic potential were evaluated for their cholesterol-lowering activity in hamsters. The strains showing the greater cholesterol removal and NPC1L1 protein down-regulation activity had no significant effects on serum and hepatic cholesterol levels in hamsters (p > 0.05). However, Lactobacillus plantarum CAAS 18008 (1 × 109 CFU/d) showing the greater bile salt deconjugation ability significantly reduced serum low-density lipoprotein cholesterol, total cholesterol, and hepatic total cholesterol levels by 28.8%, 21.7%, and 30.9%, respectively (p < 0.05). The cholesterol-lowering mechanism was attributed to its bile salt hydrolase activity, which enhanced daily fecal bile acid excretion levels and thereby accelerated new bile acid synthesis from cholesterol in liver. This study demonstrated that the strains showing greater cholesterol removal and NPC1L1 protein down-regulation activity in vitro hardly reveal cholesterol-lowering activity in vivo, whereas the strains showing greater bile salt deconjugation ability in vitro has large potential to decrease serum cholesterol levels in vivo. View Full-Text
Keywords: cholesterol removal; NPC1L1 protein; bile salt deconjugation; lactic acid bacteria; probiotic; hypocholesterolemic activity cholesterol removal; NPC1L1 protein; bile salt deconjugation; lactic acid bacteria; probiotic; hypocholesterolemic activity
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MDPI and ACS Style

Ma, C.; Zhang, S.; Lu, J.; Zhang, C.; Pang, X.; Lv, J. Screening for Cholesterol-Lowering Probiotics from Lactic Acid Bacteria Isolated from Corn Silage Based on Three Hypothesized Pathways. Int. J. Mol. Sci. 2019, 20, 2073. https://doi.org/10.3390/ijms20092073

AMA Style

Ma C, Zhang S, Lu J, Zhang C, Pang X, Lv J. Screening for Cholesterol-Lowering Probiotics from Lactic Acid Bacteria Isolated from Corn Silage Based on Three Hypothesized Pathways. International Journal of Molecular Sciences. 2019; 20(9):2073. https://doi.org/10.3390/ijms20092073

Chicago/Turabian Style

Ma, Changlu, Shuwen Zhang, Jing Lu, Cai Zhang, Xiaoyang Pang, and Jiaping Lv. 2019. "Screening for Cholesterol-Lowering Probiotics from Lactic Acid Bacteria Isolated from Corn Silage Based on Three Hypothesized Pathways" International Journal of Molecular Sciences 20, no. 9: 2073. https://doi.org/10.3390/ijms20092073

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