Phenotypic and Genotypic Characterization of West Nile Virus Isolate 2004Hou3
Abstract
:1. Introduction
2. Results
2.1. Sequencing
2.2. Pathogenesis in Mice
2.3. Viral Persistance
3. Discussion
4. Materials and Methods
4.1. Cells and Viruses
4.2. Sequencing
4.3. Animals
4.4. Viral RNA Isolation and PCR of Animal Tissue
4.5. Statistical Analyses
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
Abbreviations
DPI | Days post infection |
MPI | Months post infection |
PFU | Plaque forming units |
SNP | Single nucleotide polymorhpisms |
UTR | Untranslated region |
WNV | West Nile virus |
WNF | West Nile fever |
WNND | West Nile neuroinvasive disease |
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Nucleotide position | NY-99 nucleotide (amino acid) | 2004Hou3 nucleotide (amino acid) | Genome region |
---|---|---|---|
660 | C (I) | U (I) | prM |
774 | U (T) | C (T) | prM |
1244 | G (R) | A (K) | E |
1442 | U (V) | C (A) | E |
1465 | C (L) | U (F) | E |
1797 | C (N) | U (N) | E |
1815 | G (S) | A (S) | E |
1997 | C (A) | U (V) | E |
2049 | U (F) | C (F) | E |
2253 | A (G) | G (G) | E |
2428 | G (V) | U (F) | E |
2466 | C (H) | U (H) | E |
4107 | C (A) | U (A) | NS2A |
4146 | A (L) | G (L) | NS2A |
4803 | C (R) | U (R) | NS3 |
5275 | C (L) | U (L) | NS3 |
5280 | G (A) | A (A) | NS3 |
5736 | C (C) | U (C) | NS3 |
6045 | C (H) | U (H) | NS3 |
6138 | C (F) | U (F) | NS3 |
6238 | C (L) | U (L) | NS3 |
6405 | U (D) | C (D) | NS3 |
6426 | C (H) | U (H) | NS3 |
6589 | A (R) | C (R) | NS4A |
6726 | C (V) | U (V) | NS4A |
6996 | C (F) | U (F) | NS4B |
7416 | C (P) | U (P) | NS4B |
7938 | U (G) | C (G) | NS5 |
7977 | A (E) | G (E) | NS5 |
8199 | C (H) | U (H) | NS5 |
8235 | U (C) | C (C) | NS5 |
8325 | C (S) | U (S) | NS5 |
8520 | G (K) | A (K) | NS5 |
8727 | U (T) | C (T) | NS5 |
8835 | G (A) | A (A) | NS5 |
8970 | U (D) | C (D) | NS5 |
9013 | C (L) | U (L) | NS5 |
9144 | C (F) | U (F) | NS5 |
9352 | C (L) | U (L) | NS5 |
10,338 | C (D) | U (D) | NS5 |
10,440 | A | G | 3’UTR |
10,496 | T | C | 3’UTR |
10,703 | G | A | 3’UTR |
Days Post Infection | Brain | Lung | Heart | Spleen | Liver | Kidney | Bladder |
---|---|---|---|---|---|---|---|
47 | 4/6 | 0/6 | 0/6 | 2/6 | 1/6 | 2/6 | NA |
180 | 1/8 | 0/3 | 0/3 | 1/8 | 0/8 | 1/8 | 0/8 |
Days Post Infection | Whole Blood | Urine |
---|---|---|
47 | 5/6 | 1/6 |
60 | 3/6 | 0/6 |
90 | 3/6 | 0/6 |
180 | 2/6 | 0/6 |
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Ronca, S.E.; Gorchakov, R.; Berry, R.; Alvarado, R.E.; Gunter, S.M.; Murray, K.O. Phenotypic and Genotypic Characterization of West Nile Virus Isolate 2004Hou3. Int. J. Mol. Sci. 2019, 20, 1936. https://doi.org/10.3390/ijms20081936
Ronca SE, Gorchakov R, Berry R, Alvarado RE, Gunter SM, Murray KO. Phenotypic and Genotypic Characterization of West Nile Virus Isolate 2004Hou3. International Journal of Molecular Sciences. 2019; 20(8):1936. https://doi.org/10.3390/ijms20081936
Chicago/Turabian StyleRonca, Shannon E., Rodion Gorchakov, Rebecca Berry, R. Elias Alvarado, Sarah M. Gunter, and Kristy O. Murray. 2019. "Phenotypic and Genotypic Characterization of West Nile Virus Isolate 2004Hou3" International Journal of Molecular Sciences 20, no. 8: 1936. https://doi.org/10.3390/ijms20081936