Next Article in Journal
Therapeutic Effect of Dipsacus asperoides C. Y. Cheng et T. M. Ai in Ovalbumin-Induced Murine Model of Asthma
Previous Article in Journal
SUMOylation in Glioblastoma: A Novel Therapeutic Target
Previous Article in Special Issue
Minimally Manipulative Method for the Expansion of Human Bone Marrow Mesenchymal Stem Cells to Treat Osseous Defects
Article Menu
Issue 8 (April-2) cover image

Export Article

Open AccessArticle

Influence of Cigarette Smoke Inhalation on an Autogenous Onlay Bone Graft Area in Rats with Estrogen Deficiency: A Histomorphometric and Immunohistochemistry Study

1
Department of Diagnosis and Surgery, São Paulo State University (UNESP), School of Sciences and Technology, São José dos Campos 12245-000, Brazil
2
Department of Bioscience and Bucal Diagnosis, São Paulo State University (UNESP), School of Sciences and Technology, São José dos Campos 12245-000, Brazil
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2019, 20(8), 1854; https://doi.org/10.3390/ijms20081854
Received: 26 February 2019 / Revised: 18 March 2019 / Accepted: 21 March 2019 / Published: 15 April 2019
(This article belongs to the Special Issue Head Tissues Regeneration)
  |  
PDF [3040 KB, uploaded 15 April 2019]
  |     |  

Abstract

Purpose: The present study aimed to evaluate the influence of cigarette smoke inhalation on an autogenous onlay bone graft area, either covered with a collagen membrane or not, in healthy and estrogen-deficient rats through histomorphometry and immunohistochemistry. Materials and Methods: Sixty female rats (Wistar), weighing 250–300 g, were randomly divided and allocated into groups (either exposed to cigarette smoke inhalation or not, ovariectomized and SHAM). After 15 days, the test group underwent cigarette smoke inhalation. Sixty days after exposition, autogenous bone grafting was only performed on all right hemimandibles, and the left ones underwent autogenous onlay bone grafting with the collagen membrane (BioGide®). The graft was harvested from the parietal bone and attached to the animals’ jaws (right and left). They were euthanized at 21, 45, and 60 days after grafting. Histological measurements and immunohistochemical analyses were performed, and results were submitted to a statistical analysis. Results: The addition of a collagen membrane to the bone graft proved more efficient in preserving graft area if compared to the graft area without a collagen membrane and the one associated with cigarette smoke inhalation at 21 (p = 0.0381) and 60 days (p = 0.0192), respectively. Cigarette smoke inhalation combined with ovariectomy promoted a significant reduction of the autogenous graft area at 21 and 60 days. At 45 days, no statistically significant results were observed. In the immunohistochemical analysis, the ovariectomized and smoking subgroups, combined or not with collagen membrane, received moderate and intense immunolabeling at 21 days for Receptor Activator of Nuclear Factor Kappa-B Ligand (RANKL) (p = 0.0017 and p = 0.0381, respectively). For Osteoprotegerin (OPG), intense immunolabeling was observed in most subgroups under analysis at 60 days. Conclusion: Smoking inhalation promoted resorption on the autogenous onlay bone graft, mainly when associated with ovariectomy. Furthermore, when associated with the collagen membrane, a lower resorption rate was observed if compared to the absence of the membrane. View Full-Text
Keywords: bone regeneration; smoking; estrogen deficiency bone regeneration; smoking; estrogen deficiency
Figures

Graphical abstract

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
SciFeed

Share & Cite This Article

MDPI and ACS Style

Moretto Nunes, C.M.; Bernardo, D.V.; Ferreira, C.L.; Gomes, M.F.; De Marco, A.C.; Santamaria, M.P.; Jardini, M.A.N. Influence of Cigarette Smoke Inhalation on an Autogenous Onlay Bone Graft Area in Rats with Estrogen Deficiency: A Histomorphometric and Immunohistochemistry Study. Int. J. Mol. Sci. 2019, 20, 1854.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top