Antibiofilm Activity of Polyamide 11 Modified with Thermally Stable Polymeric Biocide Polyhexamethylene Guanidine 2-Naphtalenesulfonate
Abstract
:1. Introduction
2. Results and Discussion
2.1. FTIR Analysis of PA-11/PHMG-NS Films
2.2. Surface Properties of PA-11/PHMG-NS Films
2.3. Mechanical and Thermal Properties of PA-11/PHMG-NS Composites
2.4. Antibiofilm Efficacy of PA-11/PHMG-NS Films
2.5. Leaching Resistance of PHMG-NS from PA-11 Films
3. Materials and Methods
3.1. Materials
3.2. Synthesis of Polymeric Biocide PHMG-NS
3.3. Preparation of PA-11/PHMG-NS Composite Films
3.4. Characterisation of PA-11/PHMG-NS Composite
3.5. Biocide Release
3.6. Biofilm Assay
3.7. Bacteria Toxicity Assay
3.8. eDNA Assay
3.9. Statistical Analysis
4. Conclusions
Supplementary Materials
Author Contributions
Acknowledgments
Conflicts of Interest
Abbreviations
PA-11 | Polyamide 11 |
PHMG-Cl | Polyhexamethylene guanidine hydrochloride |
PHMG-NS | Polyhexamethylene guanidine 2-naphtalenesulfonate |
LB | Luria Broth |
MTT | Methylthiazolyldiphenyltetrazolium bromide |
eDNA | Extracellular DNA |
TE buffer | Tris-EDTA buffer |
CFU | colony forming units |
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Sample | Contact Angle (Degree) | Surface Charge Density (C/cm2) |
---|---|---|
PA-11 | 80 ± 2 | (0.47 ± 0.02) × 10−10 |
PA-11/PHMH-NS (5%) | 70 ± 2 | (1.2 ± 0.1) × 10−10 |
PA-11/PHMH-NS (7%) | 68 ± 2 | (6.5 ± 0.3) × 10−10 |
PA-11/PHMH-NS (10%) | 68 ± 2 | (27 ± 1) × 10−10 |
Sample | Tensile Strength, MPa | Elongation at Break, % |
---|---|---|
PA-11 | 45 ± 2 | 24 ± 4 |
PA-11/PHMG-NS (5%) | 45 ± 1 | 25 ± 5 |
PA-11/PHMG-NS (7%) | 43 ± 2 | 15 ± 3 |
PA-11/PHMG-NS (10%) | 27 ± 2 | 6 ± 2 |
Sample | TΔm = 5%, °C | TΔm = 10%, °C | TΔm = 20%, °C | TΔm = 50%, °C |
---|---|---|---|---|
PHMG-NS | 357 | 372 | 381 | 398 |
PA-11 | 425 | 441 | 450 | 463 |
PA-11/PHMG-NS (5%) | 413 | 434 | 445 | 456 |
PA-11/PHMG-NS (7%) | 405 | 430 | 442 | 449 |
PA-11/PHMG-NS (10%) | 391 | 408 | 420 | 443 |
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Moshynets, O.; Bardeau, J.-F.; Tarasyuk, O.; Makhno, S.; Cherniavska, T.; Dzhuzha, O.; Potters, G.; Rogalsky, S. Antibiofilm Activity of Polyamide 11 Modified with Thermally Stable Polymeric Biocide Polyhexamethylene Guanidine 2-Naphtalenesulfonate. Int. J. Mol. Sci. 2019, 20, 348. https://doi.org/10.3390/ijms20020348
Moshynets O, Bardeau J-F, Tarasyuk O, Makhno S, Cherniavska T, Dzhuzha O, Potters G, Rogalsky S. Antibiofilm Activity of Polyamide 11 Modified with Thermally Stable Polymeric Biocide Polyhexamethylene Guanidine 2-Naphtalenesulfonate. International Journal of Molecular Sciences. 2019; 20(2):348. https://doi.org/10.3390/ijms20020348
Chicago/Turabian StyleMoshynets, Olena, Jean-François Bardeau, Oksana Tarasyuk, Stanislav Makhno, Tetiana Cherniavska, Oleg Dzhuzha, Geert Potters, and Sergiy Rogalsky. 2019. "Antibiofilm Activity of Polyamide 11 Modified with Thermally Stable Polymeric Biocide Polyhexamethylene Guanidine 2-Naphtalenesulfonate" International Journal of Molecular Sciences 20, no. 2: 348. https://doi.org/10.3390/ijms20020348