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Interleukin 4 Moderately Affects Competence of Pluripotent Stem Cells for Myogenic Conversion

1
Department of Cytology, Faculty of Biology, University of Warsaw, Miecznikowa 1 Str, 02-096 Warsaw, Poland
2
Laboratory of Cytometry, Nencki Institute of Experimental Biology, Polish Academy of Sciences, Pasteur 3 Str, 02-093 Warsaw, Poland
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2019, 20(16), 3932; https://doi.org/10.3390/ijms20163932
Received: 25 July 2019 / Accepted: 8 August 2019 / Published: 13 August 2019
(This article belongs to the Special Issue Stem Cell-Based Therapy)
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Abstract

Pluripotent stem cells convert into skeletal muscle tissue during teratoma formation or chimeric animal development. Thus, they are characterized by naive myogenic potential. Numerous attempts have been made to develop protocols enabling efficient and safe conversion of pluripotent stem cells into functional myogenic cells in vitro. Despite significant progress in the field, generation of myogenic cells from pluripotent stem cells is still challenging—i.e., currently available methods require genetic modifications, animal-derived reagents, or are long lasting—and, therefore, should be further improved. In the current study, we investigated the influence of interleukin 4, a factor regulating inter alia migration and fusion of myogenic cells and necessary for proper skeletal muscle development and maintenance, on pluripotent stem cells. We assessed the impact of interleukin 4 on proliferation, selected gene expression, and ability to fuse in case of both undifferentiated and differentiating mouse embryonic stem cells. Our results revealed that interleukin 4 slightly improves fusion of pluripotent stem cells with myoblasts leading to the formation of hybrid myotubes. Moreover, it increases the level of early myogenic genes such as Mesogenin1, Pax3, and Pax7 in differentiating embryonic stem cells. Thus, interleukin 4 moderately enhances competence of mouse pluripotent stem cells for myogenic conversion. View Full-Text
Keywords: embryonic stem cells; pluripotency; differentiation; myogenesis; fusion; interleukin 4; hybrid myotubes embryonic stem cells; pluripotency; differentiation; myogenesis; fusion; interleukin 4; hybrid myotubes
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Świerczek-Lasek, B.; Neska, J.; Kominek, A.; Tolak, Ł.; Czajkowski, T.; Jańczyk-Ilach, K.; Stremińska, W.; Piwocka, K.; Ciemerych, M.A.; Archacka, K. Interleukin 4 Moderately Affects Competence of Pluripotent Stem Cells for Myogenic Conversion. Int. J. Mol. Sci. 2019, 20, 3932.

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