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Open AccessArticle

Adipose Tissue-Derived Stromal Cells in Matrigel Impact the Regeneration of Severely Damaged Skeletal Muscles

Department of Cytology, Faculty of Biology, University of Warsaw, Miecznikowa 1, 02-096 Warsaw, Poland
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Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2019, 20(13), 3313; https://doi.org/10.3390/ijms20133313
Received: 30 May 2019 / Revised: 28 June 2019 / Accepted: 1 July 2019 / Published: 5 July 2019
(This article belongs to the Special Issue Stem Cell-Based Therapy)
In case of large injuries of skeletal muscles the pool of endogenous stem cells, i.e., satellite cells, might be not sufficient to secure proper regeneration. Such failure in reconstruction is often associated with loss of muscle mass and excessive formation of connective tissue. Therapies aiming to improve skeletal muscle regeneration and prevent fibrosis may rely on the transplantation of different types of stem cell. Among such cells are adipose tissue-derived stromal cells (ADSCs) which are relatively easy to isolate, culture, and manipulate. Our study aimed to verify applicability of ADSCs in the therapies of severely injured skeletal muscles. We tested whether 3D structures obtained from Matrigel populated with ADSCs and transplanted to regenerating mouse gastrocnemius muscles could improve the regeneration. In addition, ADSCs used in this study were pretreated with myoblasts-conditioned medium or anti-TGFβ antibody, i.e., the factors modifying their ability to proliferate, migrate, or differentiate. Analyses performed one week after injury allowed us to show the impact of 3D cultured control and pretreated ADSCs at muscle mass and structure, as well as fibrosis development immune response of the injured muscle. View Full-Text
Keywords: skeletal muscle; Matrigel; ADSCs; regeneration; TGFβ skeletal muscle; Matrigel; ADSCs; regeneration; TGFβ
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Grabowska, I.; Zimowska, M.; Maciejewska, K.; Jablonska, Z.; Bazga, A.; Ozieblo, M.; Streminska, W.; Bem, J.; Brzoska, E.; Ciemerych, M.A. Adipose Tissue-Derived Stromal Cells in Matrigel Impact the Regeneration of Severely Damaged Skeletal Muscles. Int. J. Mol. Sci. 2019, 20, 3313.

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