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Open AccessArticle

JA-Induced Endocytosis of AtRGS1 Is Involved in G-Protein Mediated JA Responses

by Li Li 1,†, Bodan Su 1,†, Xueying Qi 1, Xi Zhang 1, Susheng Song 2 and Xiaoyi Shan 1,*
1
College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 10083, China
2
Beijing Key Laboratory of Plant Gene Resources and Biotechnology for Carbon Reduction and Environmental Improvement, College of Life Sciences, Capital Normal University, Beijing 100048, China
*
Author to whom correspondence should be addressed.
These authors contributed equally to this article.
Int. J. Mol. Sci. 2019, 20(15), 3779; https://doi.org/10.3390/ijms20153779
Received: 28 June 2019 / Revised: 27 July 2019 / Accepted: 29 July 2019 / Published: 2 August 2019
(This article belongs to the Special Issue Jasmonic Acid Pathway in Plants)
Arabidopsis heterotrimeric G proteins regulate diverse plant growth and defense processes by coupling to 7TM AtRGS1 proteins. Although G protein mutants display alterations in response to multiple plant hormones, the underlying mechanism by which G proteins participate in the regulation of hormone responses remains elusive. Here, we show that genetic disruption of Gα and Gβ subunits results in reduced sensitivity to JA treatment. Furthermore, using confocal microscopy, VA-TIRFM, and FRET-FLIM, we provide evidence that stimulation by JA induces phosphorylation- and C-terminus-dependent endocytosis of AtRGS1, which then promotes dissociation of AtRGS1 from AtGPA1. In addition, SPT analysis reveals that JA treatment affects the diffusion dynamics of AtRGS1 and AtRGS1-ΔCt. Taken together, these findings suggest that the JA signal activates heterotrimeric G proteins through the endocytosis of AtRGS1 and dissociation of AtRGS1 from AtGPA1, thus providing valuable insight into the mechanisms of how the G protein system perceives and transduces phytohormone signals. View Full-Text
Keywords: heterotrimeric G proteins; AtRGS1; jasmonates; endocytosis; diffusion dynamics heterotrimeric G proteins; AtRGS1; jasmonates; endocytosis; diffusion dynamics
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Li, L.; Su, B.; Qi, X.; Zhang, X.; Song, S.; Shan, X. JA-Induced Endocytosis of AtRGS1 Is Involved in G-Protein Mediated JA Responses. Int. J. Mol. Sci. 2019, 20, 3779.

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