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Article

BrTCP7 Transcription Factor Is Associated with MeJA-Promoted Leaf Senescence by Activating the Expression of BrOPR3 and BrRCCR

1
State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources/Guangdong Provincial Key Laboratory of Postharvest Science of Fruits and Vegetables/Engineering Research Center of Southern Horticultural Products Preservation, Ministry of Education, College of Horticulture, South China Agricultural University, Guangzhou 510642, China
2
Scientific Observing and Experimental Station of Crop Cultivation in South China, Ministry of Agriculture, College of Agriculture, South China Agricultural University, Guangzhou 510642, China
3
Department of Food Science, Guangdong Food and Drug Vocational College, Guangzhou 510520, China
*
Authors to whom correspondence should be addressed.
Int. J. Mol. Sci. 2019, 20(16), 3963; https://doi.org/10.3390/ijms20163963
Received: 21 June 2019 / Revised: 11 August 2019 / Accepted: 13 August 2019 / Published: 14 August 2019
(This article belongs to the Special Issue Jasmonic Acid Pathway in Plants)
The plant hormone jasmonic acid (JA) has been recognized as an important promoter of leaf senescence in plants. However, upstream transcription factors (TFs) that control JA biosynthesis during JA-promoted leaf senescence remain unknown. In this study, we report the possible involvement of a TEOSINTE BRANCHED1/CYCLOIDEA/PCF (TCP) TF BrTCP7 in methyl jasmonate (MeJA)-promoted leaf senescence in Chinese flowering cabbage. Exogenous MeJA treatment reduced maximum quantum yield (Fv/Fm) and total chlorophyll content, accompanied by the increased expression of senescence marker and chlorophyll catabolic genes, and accelerated leaf senescence. To further understand the transcriptional regulation of MeJA-promoted leaf senescence, a class I member of TCP TFs BrTCP7 was examined. BrTCP7 is a nuclear protein and possesses trans-activation ability through subcellular localization and transcriptional activity assays. A higher level of BrTCP7 transcript was detected in senescing leaves, and its expression was up-regulated by MeJA. The electrophoretic mobility shift assay and transient expression assay showed that BrTCP7 binds to the promoter regions of a JA biosynthetic gene BrOPR3 encoding OPDA reductase3 (OPR3) and a chlorophyll catabolic gene BrRCCR encoding red chlorophyll catabolite reductase (RCCR), activating their transcriptions. Taken together, these findings reveal that BrTCP7 is associated with MeJA-promoted leaf senescence at least partly by activating JA biosynthesis and chlorophyll catabolism, thus expanding our knowledge of the transcriptional mechanism of JA-mediated leaf senescence. View Full-Text
Keywords: Chinese flowering cabbage; leaf senescence; JA; transcriptional activation Chinese flowering cabbage; leaf senescence; JA; transcriptional activation
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MDPI and ACS Style

Xu, Y.-m.; Xiao, X.-m.; Zeng, Z.-x.; Tan, X.-l.; Liu, Z.-l.; Chen, J.-w.; Su, X.-g.; Chen, J.-y. BrTCP7 Transcription Factor Is Associated with MeJA-Promoted Leaf Senescence by Activating the Expression of BrOPR3 and BrRCCR. Int. J. Mol. Sci. 2019, 20, 3963. https://doi.org/10.3390/ijms20163963

AMA Style

Xu Y-m, Xiao X-m, Zeng Z-x, Tan X-l, Liu Z-l, Chen J-w, Su X-g, Chen J-y. BrTCP7 Transcription Factor Is Associated with MeJA-Promoted Leaf Senescence by Activating the Expression of BrOPR3 and BrRCCR. International Journal of Molecular Sciences. 2019; 20(16):3963. https://doi.org/10.3390/ijms20163963

Chicago/Turabian Style

Xu, Yan-mei; Xiao, Xian-mei; Zeng, Ze-xiang; Tan, Xiao-li; Liu, Zong-li; Chen, Jian-wen; Su, Xin-guo; Chen, Jian-ye. 2019. "BrTCP7 Transcription Factor Is Associated with MeJA-Promoted Leaf Senescence by Activating the Expression of BrOPR3 and BrRCCR" Int. J. Mol. Sci. 20, no. 16: 3963. https://doi.org/10.3390/ijms20163963

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