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Open AccessCommunication

Identification of Jasmonic Acid Biosynthetic Genes in Sweet Cherry and Expression Analysis in Four Ancient Varieties from Tuscany

1
University of Siena, Department of Life Sciences, via P.A. Mattioli 4, 53100 Siena, Italy
2
Trees and timber institute-National research council of Italy (CNR-IVALSA), via Aurelia 49, 58022 Follonica (GR), Italy
3
Research and Innovation Department, Luxembourg Institute of Science and Technology, 5 avenue des Hauts-Fourneaux, L-4362 Esch/Alzette, Luxembourg
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2019, 20(14), 3569; https://doi.org/10.3390/ijms20143569
Received: 26 June 2019 / Revised: 15 July 2019 / Accepted: 18 July 2019 / Published: 22 July 2019
(This article belongs to the Special Issue Jasmonic Acid Pathway in Plants)
Sweet cherries are non-climacteric fruits whose early development is characterized by high levels of the phytohormone jasmonic acid (JA). Important parameters, such as firmness and susceptibility to cracking, can be affected by pre- and postharvest treatments of sweet cherries with JA. Despite the impact of JA on sweet cherry development and fruit characteristics, there are no studies (to the best of our knowledge) identifying the genes involved in the JA biosynthetic pathway in this species. We herein identify the sweet cherry members of the lipoxygenase family (13-LOX); allene oxide synthase, allene oxide cyclase and 12-oxo-phytodienoic acid reductase 3, as well as genes encoding the transcriptional master regulator MYC2. We analyze their expression pattern in four non-commercial Tuscan varieties (‘Carlotta’, ‘Maggiola’, ‘Morellona’, ‘Crognola’) having different levels of bioactives (namely phenolics). The highest differences are found in two genes encoding 13-LOX in the variety ‘Maggiola’ and one MYC2 isoform in ‘Morellona’. No statistically-significant variations are instead present in the allene oxide synthase, allene oxide cyclase and 12-oxo-phytodienoic acid reductase 3. Our data pave the way to follow-up studies on the JA signaling pathway in these ancient varieties, for example in relation to development and post-harvest storage. View Full-Text
Keywords: Prunus avium; Tuscan varieties; jasmonic acid; lipoxygenase; bioinformatics; gene expression Prunus avium; Tuscan varieties; jasmonic acid; lipoxygenase; bioinformatics; gene expression
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MDPI and ACS Style

Berni, R.; Cai, G.; Xu, X.; Hausman, J.-F.; Guerriero, G. Identification of Jasmonic Acid Biosynthetic Genes in Sweet Cherry and Expression Analysis in Four Ancient Varieties from Tuscany. Int. J. Mol. Sci. 2019, 20, 3569.

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