The Respiratory Burst Oxidase Homolog D (RbohD) Cell and Tissue Distribution in Potato–Potato Virus Y (PVYNTN) Hypersensitive and Susceptible Reactions
Abstract
:1. Introduction
2. Results
2.1. Immunofluorescence Localization of RbohD Protein in Compatible and Incompatible Interactions
2.2. Ultrastructural Localization of RbohD Protein in Compatible and Incompatible Interactions
2.3. Detection of H2O2 and Virus Particles Location in Susceptible and Hypersensitive Reaction to PVYNTN
3. Discussion
4. Material and Methods
4.1. Plants and Virus
4.2. Immunofluorescence Localization of RbohD and the Assessment of the Quantitative Fluorescence Signal
4.3. Ultrastructural Virus Particles Location and Hydrogen Peroxide Detection
4.4. Ultrastructural Localization of RbohD and Quantitative Immunogold Localization by Direct Estimation of the Relative Labeling Index (RLI)
5. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Sample | Parameters of Immunogold Labeling | ||||
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G0 | Ge | RLI | Χ2 Value | Χ2 as % | |
Immunogold Localization of RbohD in Potato Sárpo Mira (Resistant): | |||||
1. Mock-inoculated potato Sárpo Mira plants | |||||
cell wall | 1 | 1 | 1.00 | 0.00 | 0.00 |
nucleus | 8 | 6 | 1.33 | 0.67 | 0.55 |
endoplasmatic reticulum (ER) | 2 | 6 | 0.33 | 2.67 | 2.19 |
Golgi apparatus | 10 | 2 | 5.00 * | 32.00 | 26.28 * |
chloroplasts | 10 | 2 | 5.00 * | 32.00 | 26.28 * |
mitochondrion | 1 | 5 | 0.20 | 3.20 | 2.63 |
vacuole | 30 | 9 | 3.33 * | 49.00 | 40.24 * |
cytoplasm | 1 | 4 | 0.25 | 2.25 | 1.85 |
vesicles | 10 | 3 | 3.33 * | 16.33 | 13.41 * |
Column total | 121.78 | ||||
2. 1 day after PVYNTN-inoculation potato Sárpo Mira plants | |||||
cell wall | 30 | 7 | 4.29 * | 75.57 | 47.72 * |
nucleus | 8 | 6 | 1.33 | 0.67 | 0.42 |
endoplasmic reticulum (ER) | 2 | 6 | 0.33 | 2.67 | 1.68 |
Golgi apparatus | 10 | 2 | 5.00 * | 32.00 | 20.21 * |
chloroplasts | 12 | 6 | 2.00 | 6.00 | 3.79 |
mitochondrion | 1 | 5 | 0.20 | 3.20 | 2.02 |
vacuole | 27 | 9 | 3.00 * | 36.00 | 22.73 * |
cytoplasm | 1 | 4 | 0.25 | 2.25 | 1.42 |
vesicles | 32 | 7 | 4.57 * | 89.29 | 56.38 * |
Column total | 158.35 | ||||
3. 7 days after PVYNTN inoculation potato Sárpo Mira plants | |||||
cell wall | 40 | 10 | 4.00 * | 90.00 | 50.62 * |
nucleus | 18 | 6 | 3.00 * | 24.00 | 13.50 * |
endoplasmic reticulum (ER) | 2 | 6 | 0.33 | 2.67 | 1.50 |
Golgi apparatus | 12 | 2 | 6.00 * | 50.00 | 28.12 * |
chloroplasts | 24 | 19 | 1.26 | 1.32 | 0.74 |
mitochondrion | 4 | 5 | 0.80 | 0.20 | 0.11 |
vacuole | 12 | 6 | 2.00 | 6.00 | 3.37 |
cytoplasm | 16 | 10 | 1.60 | 3.60 | 2.02 |
vesicles | 39 | 10 | 3.90 * | 84.10 | 47.31 * |
Column total | 177.78 |
Sample | Parameters of Immunogold Labeling | ||||
---|---|---|---|---|---|
G0 | Ge | RLI | Χ2 Value | Χ2 as % | |
Immunogold Localization of RbohD in Potato Rywal (Less Resistant): | |||||
1. Mock-inoculated potato Rywal plants | |||||
cell wall | 5 | 3 | 1.67 | 1.33 | 1.02 |
nucleus | 4 | 3 | 1.33 | 0.33 | 0.25 |
endoplasmic reticulum (ER) | 4 | 3 | 1.33 | 0.33 | 0.25 |
Golgi apparatus | 12 | 2 | 6.00 * | 50.00 | 38.17 * |
chloroplasts | 9 | 2 | 4.50 * | 24.50 | 18.71 * |
mitochondrion | 1 | 7 | 0.14 | 5.14 | 3.93 |
vacuole | 10 | 3 | 3.33 * | 16.33 | 24.43 * |
cytoplasm | 2 | 4 | 0.50 | 1.00 | 0.76 |
vesicles | 10 | 2 | 5.00 * | 32.00 | 24.43 * |
Column total | 130.98 | ||||
2. 1 day after PVYNTN-inoculation potato Rywal plants | |||||
cell wall | 24 | 9 | 2.67 * | 25.00 | 13.88 * |
nucleus | 20 | 6 | 3.33 * | 32.67 | 18.14 * |
endoplasmic reticulum (ER) | 2 | 6 | 0.33 | 2.67 | 1.48 |
Golgi apparatus | 10 | 2 | 5.00 * | 32.00 | 17.77 * |
chloroplasts | 12 | 3 | 4.00 * | 27.00 | 14.99 * |
mitochondrion | 14 | 3 | 4.67 * | 40.33 | 22.39 * |
vacuole | 29 | 9 | 3.22 * | 44.44 | 24.68 * |
cytoplasm | 6 | 4 | 1.50 | 1.00 | 0.56 |
vesicles | 7 | 6 | 1.17 | 0.17 | 0.09 |
Column total | 180.11 | ||||
3. 3 days after PVYNTN-inoculation potato Rywal plants | |||||
cell wall | 24 | 9 | 2.67 | 25.00 | 8.76 |
nucleus | 8 | 6 | 1.33 | 0.67 | 0.23 |
endoplasmic reticulum (ER) | 2 | 6 | 0.33 | 2.67 | 0.93 |
Golgi apparatus | 10 | 2 | 5.00 * | 32.00 | 11.22 * |
chloroplasts | 45 | 10 | 4.50 * | 122.50 | 42.94 * |
mitochondrion | 24 | 5 | 4.80 * | 72.20 | 25.31 * |
vacuole | 30 | 9 | 3.33 * | 49.00 | 17.18 * |
cytoplasm | 9 | 4 | 2.25 | 6.25 | 2.19 |
vesicles | 6 | 5 | 1.20 | 0.20 | 0.07 |
Column total | 285.28 |
Sample | Parameters of Immunogold Labeling | ||||
---|---|---|---|---|---|
G0 | Ge | RLI | Χ2 Value | Χ2 as % | |
Immunogold localization of RbohD in potato Irys (susceptible): | |||||
1. Mock-inoculated potato Irys plants | |||||
cell wall | 5 | 1 | 5.00 | 16.00 | 9.49 |
nucleus | 1 | 2 | 0.50 | 0.50 | 0.30 |
endoplasmic reticulum (ER) | 2 | 6 | 0.33 | 2.67 | 1.58 |
Golgi apparatus | 10 | 1 | 10.00 * | 81.00 | 48.02 * |
chloroplasts | 7 | 1 | 7.00 * | 36.00 | 21.34 * |
mitochondrion | 1 | 1 | 1.00 | 0.00 | 0.00 |
vacuole | 1 | 2 | 0.50 | 0.50 | 0.30 |
cytoplasm | 10 | 2 | 5.00 * | 32.00 | 18.97 * |
vesicles | 1 | 2 | 0.50 | 0.50 | 0.30 |
Column total | 168.67 | ||||
2. 7 days after PVYNTN-inoculation potato Irys plants | |||||
cell wall | 19 | 5 | 3.80 | 39.20 | 7.65 |
nucleus | 1 | 2 | 0.50 | 0.50 | 0.10 |
endoplasmic reticulum (ER) | 2 | 6 | 0.33 | 2.67 | 0.52 |
Golgi apparatus | 10 | 1 | 10.00 * | 81.00 | 15.80 * |
chloroplasts | 30 | 3 | 10.00 * | 243.00 | 47.41 * |
mitochondrion | 25 | 5 | 5.00 * | 80.00 | 15.61 * |
vacuole | 23 | 5 | 4.60 * | 64.80 | 12.64 * |
cytoplasm | 5 | 3 | 1.67 | 1.33 | 0.26 |
vesicles | 32 | 16 | 2.00 | 16.00 | 3.12 |
Column total | 512.50 | ||||
3. 21 days after PVYNTN inoculation potato Irys plants | |||||
cell wall | 15 | 5 | 3.00 | 20.00 | 4.60 |
nucleus | 1 | 2 | 0.50 | 0.50 | 0.11 |
endoplasmic reticulum (ER) | 1 | 5 | 0.20 | 3.20 | 0.74 |
Golgi apparatus | 5 | 1 | 5.00 | 16.00 | 3.68 |
chloroplasts | 22 | 2 | 11.00 * | 200.00 | 45.99 * |
mitochondrion | 2 | 5 | 0.40 | 1.80 | 0.41 |
vacuole | 10 | 2 | 5.00 | 32.00 | 7.36 |
cytoplasm | 25 | 3 | 8.33 * | 161.33 | 37.10 * |
vesicles | 12 | 5 | 2.40 | 9.80 | 2.25 |
Column total | 434.83 |
© 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
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Otulak-Kozieł, K.; Kozieł, E.; Valverde, R.A. The Respiratory Burst Oxidase Homolog D (RbohD) Cell and Tissue Distribution in Potato–Potato Virus Y (PVYNTN) Hypersensitive and Susceptible Reactions. Int. J. Mol. Sci. 2019, 20, 2741. https://doi.org/10.3390/ijms20112741
Otulak-Kozieł K, Kozieł E, Valverde RA. The Respiratory Burst Oxidase Homolog D (RbohD) Cell and Tissue Distribution in Potato–Potato Virus Y (PVYNTN) Hypersensitive and Susceptible Reactions. International Journal of Molecular Sciences. 2019; 20(11):2741. https://doi.org/10.3390/ijms20112741
Chicago/Turabian StyleOtulak-Kozieł, Katarzyna, Edmund Kozieł, and Rodrigo A. Valverde. 2019. "The Respiratory Burst Oxidase Homolog D (RbohD) Cell and Tissue Distribution in Potato–Potato Virus Y (PVYNTN) Hypersensitive and Susceptible Reactions" International Journal of Molecular Sciences 20, no. 11: 2741. https://doi.org/10.3390/ijms20112741