Next Article in Journal
LT-IIc, A Bacterial Type II Heat-Labile Enterotoxin, Induces Specific Lethality in Triple Negative Breast Cancer Cells by Modulation of Autophagy and Induction of Apoptosis and Necroptosis
Previous Article in Journal
Shot-Gun Proteomic Analysis on Roots of Arabidopsis pldα1 Mutants Suggesting the Involvement of PLDα1 in Mitochondrial Protein Import, Vesicular Trafficking and Glucosinolate Biosynthesis
Open AccessArticle

The Unique Mechanisms of Cellular Proliferation, Migration and Apoptosis are Regulated through Oocyte Maturational Development—A Complete Transcriptomic and Histochemical Study

1
Division of Infertility and Reproductive Endocrinology, Department of Gynecology, Obstetrics and Gynecological Oncology, Poznan University of Medical Sciences, 60-535 Poznań, Poland
2
Department of Obstetrics and Gynecology, University Hospital and Masaryk University, 601 77 Brno, Czech Republic
3
Department of Histology and Embryology, Poznan University of Medical Sciences, 60-781 Poznań, Poland
4
Department of Anatomy, Poznan University of Medical Sciences, 60-781 Poznań, Poland
5
Veterinary Center, Nicolaus Copernicus University in Torun, 87-100 Toruń, Poland
6
Department of Histology and Embryology, Wroclaw University of Medical Sciences, 50-368 Wrocław, Poland
7
Division of Anatomy and Histology, University of Zielona Gora, 65-046 Zielona Góra, Poland
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2019, 20(1), 84; https://doi.org/10.3390/ijms20010084
Received: 5 November 2018 / Revised: 6 December 2018 / Accepted: 21 December 2018 / Published: 26 December 2018
(This article belongs to the Section Molecular Biology)
The growth and development of oocyte affect the functional activities of the surrounding somatic cells. These cells are regulated by various types of hormones, proteins, metabolites, and regulatory molecules through gap communication, ultimately leading to the development and maturation of oocytes. The close association between somatic cells and oocytes, which together form the cumulus-oocyte complexes (COCs), and their bi-directional communication are crucial for the acquisition of developmental competences by the oocyte. In this study, oocytes were extracted from the ovaries obtained from crossbred landrace gilts and subjected to in vitro maturation. RNA isolated from those oocytes was used for the subsequent microarray analysis. The data obtained shows, for the first time, variable levels of gene expression (fold changes higher than |2| and adjusted p-value < 0.05) belonging to four ontological groups: regulation of cell proliferation (GO:0042127), regulation of cell migration (GO:0030334), and regulation of programmed cell death (GO:0043067) that can be used together as proliferation, migration or apoptosis markers. We have identified several genes of porcine oocytes (ID2, VEGFA, BTG2, ESR1, CCND2, EDNRA, ANGPTL4, TGFBR3, GJA1, LAMA2, KIT, TPM1, VCP, GRID2, MEF2C, RPS3A, PLD1, BTG3, CD47, MITF), whose expression after in vitro maturation (IVM) is downregulated with different degrees. Our results may be helpful in further elucidating the molecular basis and functional significance of a number of gene markers associated with the processes of migration, proliferation and angiogenesis occurring in COCs. View Full-Text
Keywords: pig; oocytes; microarray; cellular competence pig; oocytes; microarray; cellular competence
Show Figures

Figure 1

MDPI and ACS Style

Chermuła, B.; Brązert, M.; Jeseta, M.; Ożegowska, K.; Sujka-Kordowska, P.; Konwerska, A.; Bryja, A.; Kranc, W.; Jankowski, M.; Nawrocki, M.J.; Kocherova, I.; Celichowski, P.; Borowiec, B.; Popis, M.; Budna-Tukan, J.; Antosik, P.; Bukowska, D.; Brussow, K.P.; Pawelczyk, L.; Bruska, M.; Zabel, M.; Nowicki, M.; Kempisty, B. The Unique Mechanisms of Cellular Proliferation, Migration and Apoptosis are Regulated through Oocyte Maturational Development—A Complete Transcriptomic and Histochemical Study. Int. J. Mol. Sci. 2019, 20, 84.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop