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Int. J. Mol. Sci. 2019, 20(1), 82; https://doi.org/10.3390/ijms20010082

Shot-Gun Proteomic Analysis on Roots of Arabidopsis pldα1 Mutants Suggesting the Involvement of PLDα1 in Mitochondrial Protein Import, Vesicular Trafficking and Glucosinolate Biosynthesis

1
Centre of the Region Haná for Biotechnological and Agricultural Research, Faculty of Science, Palacký University, Šlechtitelů 27, 78371 Olomouc, Czech Republic
2
Institute for Genomics, Biocomputing & Biotechnology, Mississippi Agricultural and Forestry Experiment Station, Mississippi State University, Starkville, MS 39759, USA
*
Author to whom correspondence should be addressed.
Received: 19 November 2018 / Revised: 19 December 2018 / Accepted: 21 December 2018 / Published: 26 December 2018
(This article belongs to the Section Molecular Plant Sciences)
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Abstract

Phospholipase Dα1 (PLDα1) belongs to phospholipases, a large phospholipid hydrolyzing protein family. PLDα1 has a substrate preference for phosphatidylcholine leading to enzymatic production of phosphatidic acid, a lipid second messenger with multiple cellular functions. PLDα1 itself is implicated in biotic and abiotic stress responses. Here, we present a shot-gun differential proteomic analysis on roots of two Arabidopsis pldα1 mutants compared to the wild type. Interestingly, PLDα1 deficiency leads to altered abundances of proteins involved in diverse processes related to membrane transport including endocytosis and endoplasmic reticulum-Golgi transport. PLDα1 may be involved in the stability of attachment sites of endoplasmic reticulum to the plasma membrane as suggested by increased abundance of synaptotagmin 1, which was validated by immunoblotting and whole-mount immunolabelling analyses. Moreover, we noticed a robust abundance alterations of proteins involved in mitochondrial import and electron transport chain. Notably, the abundances of numerous proteins implicated in glucosinolate biosynthesis were also affected in pldα1 mutants. Our results suggest a broader biological involvement of PLDα1 than anticipated thus far, especially in the processes such as endomembrane transport, mitochondrial protein import and protein quality control, as well as glucosinolate biosynthesis. View Full-Text
Keywords: phospholipase D alpha1; Arabidopsis; proteomics; mitochondrial protein import; quality control; vesicular transport; cytoskeleton phospholipase D alpha1; Arabidopsis; proteomics; mitochondrial protein import; quality control; vesicular transport; cytoskeleton
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Takáč, T.; Šamajová, O.; Vadovič, P.; Pechan, T.; Šamaj, J. Shot-Gun Proteomic Analysis on Roots of Arabidopsis pldα1 Mutants Suggesting the Involvement of PLDα1 in Mitochondrial Protein Import, Vesicular Trafficking and Glucosinolate Biosynthesis. Int. J. Mol. Sci. 2019, 20, 82.

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