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Expression of Vitis amurensis VaERF20 in Arabidopsis thaliana Improves Resistance to Botrytis cinerea and Pseudomonas syringae pv. Tomato DC3000

by Mengnan Wang 1,2, Yanxun Zhu 1,2, Rui Han 1,2, Wuchen Yin 1,2, Chunlei Guo 1,2, Zhi Li 1,2 and Xiping Wang 1,2,*
1
State Key Laboratory of Crop Stress Biology in Arid Areas, College of Horticulture, Northwest A&F University, Yangling 712100, China
2
Key Laboratory of Horticultural Plant Biology and Germplasm Innovation in Northwest China, Ministry of Agriculture, Yangling 712100, China
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2018, 19(3), 696; https://doi.org/10.3390/ijms19030696
Received: 10 January 2018 / Revised: 2 February 2018 / Accepted: 6 February 2018 / Published: 1 March 2018
(This article belongs to the Special Issue Plant Defense Genes Against Biotic Stresses)
Ethylene response factor (ERF) transcription factors play important roles in regulating immune responses in plants. In our study, we characterized a member of the ERF transcription factor family, VaERF20, from the Chinese wild Vitis genotype, V. amurensis Rupr “Shuangyou”. Phylogenetic analysis indicated that VaERF20 belongs to group IXc of the ERF family, in which many members are known to contribute to fighting pathogen infection. Consistent with this, expression of VaERF20 was induced by treatment with the necrotrophic fungal pathogen Botrytis cinerea (B. cinerea) in “Shuangyou” and V. vinifera “Red Globe”. Arabidopsis thaliana plants over-expressing VaERF20 displayed enhanced resistance to B. cinerea and the bacterium Pseudomonas syringae pv. tomato (Pst) DC3000. Patterns of pathogen-induced reactive oxygen species (ROS) accumulation were entirely distinct in B. cinerea and PstDC3000 inoculated plants. Examples of both salicylic acid (SA) and jasmonic acid/ethylene (JA/ET) responsive defense genes were up-regulated after B. cinerea and PstDC3000 inoculation of the VaERF20-overexpressing transgenic A. thaliana plants. Evidence of pattern-triggered immunity (PTI), callose accumulation and stomatal defense, together with increased expression of PTI genes, was also greater in the transgenic lines. These data indicate that VaERF20 participates in various signal transduction pathways and acts as an inducer of immune responses. View Full-Text
Keywords: VaERF20; grapevine; Botrytis cinerea; Pseudomonas syringae pv. tomato DC3000 VaERF20; grapevine; Botrytis cinerea; Pseudomonas syringae pv. tomato DC3000
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Wang, M.; Zhu, Y.; Han, R.; Yin, W.; Guo, C.; Li, Z.; Wang, X. Expression of Vitis amurensis VaERF20 in Arabidopsis thaliana Improves Resistance to Botrytis cinerea and Pseudomonas syringae pv. Tomato DC3000. Int. J. Mol. Sci. 2018, 19, 696.

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