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Functional and Molecular Changes of the Bladder in Rats with Crushing Injury of Nerve Bundles from Major Pelvic Ganglion to the Bladder: Role of RhoA/Rho Kinase Pathway

1
Department of Urology, Seoul St. Mary's Hospital, the Catholic University of Korea, 505 Banpo-dong, Seocho-gu, Seoul 137-701, Korea
2
Department of Urology, St. Vincent's Hospital, the Catholic University of Korea, 93 Jungbu-daero, Paldal-gu, Suwon 442-723, Gyeonggi-do, Korea
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2013, 14(9), 17511-17524; https://doi.org/10.3390/ijms140917511
Received: 8 June 2013 / Revised: 20 July 2013 / Accepted: 5 August 2013 / Published: 27 August 2013
Voiding dysfunction is a common complication after radical pelvic surgery. To reduce this complication, nerve-sparing radical pelvic surgery was introduced. However, several patients experienced voiding difficulty despite nerve-sparing radical pelvic surgery. Thus, we investigated the functional and molecular changes of the bladder in rats, which demonstrated voiding dysfunction induced by nerve damage during nerve-sparing radical pelvic surgery. Male rats were used and assigned to normal, sham-operated, and bilateral crushing nerve bundles from major pelvic ganglion (MPG) to bladder group. After one, two, and four-week crushing injury, significantly decreased contractile response and increased connective tissue of the detrusor were observed and these results were reliable findings with voiding difficulty following nerve-sparing radical pelvic surgery. After crushing injury, significantly increased M2 muscarinic receptor expression was observed and this might be regarded as the compensatory response. However, M3 muscarinic receptor expression was not significantly changed. The expression of RhoA, ROCK-α, and ROCK-β was significantly increased after one, two, and four-week crushing injury. From these results, the down-regulation of RhoA/Rho kinase pathway might lead to the decreased bladder contractility after crushing injury of nerve bundles from MPG to the bladder despite of the compensated up-regulation of M2 muscarinic receptor. View Full-Text
Keywords: urinary bladder; receptor; muscarinic; Rho-associated kinase; autonomic ganglia; injury; nerve crush; rat urinary bladder; receptor; muscarinic; Rho-associated kinase; autonomic ganglia; injury; nerve crush; rat
MDPI and ACS Style

Kim, S.J.; Lee, D.S.; Bae, W.J.; Kim, S.; Hong, S.H.; Lee, J.Y.; Hwang, T.-K.; Kim, S.W. Functional and Molecular Changes of the Bladder in Rats with Crushing Injury of Nerve Bundles from Major Pelvic Ganglion to the Bladder: Role of RhoA/Rho Kinase Pathway. Int. J. Mol. Sci. 2013, 14, 17511-17524.

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