Next Article in Journal
Mechanistic Advances in Hypoglycemic Effects of Natural Polysaccharides: Multi-Target Regulation of Glycometabolism and Gut Microbiota Crosstalk
Previous Article in Journal
The Synthesis and Property Study of NH-Ac-Anchored Multilayer 3D Polymers
Previous Article in Special Issue
Exploring the Structural Characteristics and Antioxidant Capacity of Pectins from Adenophora tetraphylla (Thunb.) Fisch.
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Molecules
Volume 30
Issue 9
10.3390/molecules30091979
molecules-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
This is an early access version, the complete PDF, HTML, and XML versions will be available soon.
Article

Tyrosinase Inhibitors Among Flora of Lubelskie Region—Application of Bio-Chromatographic Approach and Zebrafish Model in Bioactivity Screening of Plant Material

by
Kamila Kusio-Targońska
1,
Nataliia Kosheva
2,
Krzysztof Kamil Wojtanowski
1,
Katarzyna Gaweł-Bęben
3,
Dimitris Beis
4 and
Wirginia Kukula-Koch
1,*
1
Department of Pharmacognosy with Medicinal Plants Garden, Medical University of Lublin, 20-093 Lublin, Poland
2
Department of Experimental and Clinical Pharmacology, Medical University of Lublin, 20-090 Lublin, Poland
3
Department of Cosmetology, The University of Information Technology and Management in Rzeszow, 35-225 Rzeszow, Poland
4
Laboratory of Biological Chemistry, Faculty of Medicine, School of Health Sciences, University of Ioannina, 45110 Ioannina, Greece
*
Author to whom correspondence should be addressed.
Molecules 2025, 30(9), 1979; https://doi.org/10.3390/molecules30091979
Submission received: 18 March 2025 / Revised: 25 April 2025 / Accepted: 28 April 2025 / Published: 29 April 2025
(This article belongs to the Special Issue Medicinal Value of Natural Bioactive Compounds and Plant Extracts, 3rd Edition)
Versions Notes

Abstract

The whitening potential of natural products is commonly assessed through spectrophotometric assays that colorimetrically measure the inhibitory effects on tyrosinase, a key enzyme in pigment formation. However, these assays fail to provide evidence about the input of individual components into the total activity of a mixture like plant extracts. This study introduced chromatographic methods to identify active natural products without isolating them from their mixtures. In this study, various plant extracts of differing polarities (EtOH, 50% EtOH, and HOH) from species growing in the Lubelskie region of Poland were evaluated for their ability to inhibit tyrosinase. The most active extract identified through spectrophotometric assays was a 50% EtOH extract from Matricaria recutita L. (Chamomilla recutita (L.) Rauschert). Subsequent HPLC-MS analysis allowed for the identification of several active compounds from different classes, including organic acids, glycosylated phenolics, and phenolic acids that interacted with the enzyme. The bioactivity of individual components was confirmed through classical spectrophotometric assays, highlighting ferulic acid (IC50 = 0.484 µM), quinic acid (IC50 = 22.90 µM), and citric acid (IC50 = 24.18 µM) as three representatives of different classes of molecules with inhibitory potential. Furthermore, the whitening capacity of the chamomile extract was investigated in a zebrafish model, demonstrating effective pigmentation inhibition in Danio rerio larvae and validating the proposed chromatographic approach.
Keywords: chamomile; cosmetic properties; bioassays; HPLC-MS; plants chamomile; cosmetic properties; bioassays; HPLC-MS; plants

Share and Cite

MDPI and ACS Style

Kusio-Targońska, K.; Kosheva, N.; Wojtanowski, K.K.; Gaweł-Bęben, K.; Beis, D.; Kukula-Koch, W. Tyrosinase Inhibitors Among Flora of Lubelskie Region—Application of Bio-Chromatographic Approach and Zebrafish Model in Bioactivity Screening of Plant Material. Molecules 2025, 30, 1979. https://doi.org/10.3390/molecules30091979

AMA Style

Kusio-Targońska K, Kosheva N, Wojtanowski KK, Gaweł-Bęben K, Beis D, Kukula-Koch W. Tyrosinase Inhibitors Among Flora of Lubelskie Region—Application of Bio-Chromatographic Approach and Zebrafish Model in Bioactivity Screening of Plant Material. Molecules. 2025; 30(9):1979. https://doi.org/10.3390/molecules30091979

Chicago/Turabian Style

Kusio-Targońska, Kamila, Nataliia Kosheva, Krzysztof Kamil Wojtanowski, Katarzyna Gaweł-Bęben, Dimitris Beis, and Wirginia Kukula-Koch. 2025. "Tyrosinase Inhibitors Among Flora of Lubelskie Region—Application of Bio-Chromatographic Approach and Zebrafish Model in Bioactivity Screening of Plant Material" Molecules 30, no. 9: 1979. https://doi.org/10.3390/molecules30091979

APA Style

Kusio-Targońska, K., Kosheva, N., Wojtanowski, K. K., Gaweł-Bęben, K., Beis, D., & Kukula-Koch, W. (2025). Tyrosinase Inhibitors Among Flora of Lubelskie Region—Application of Bio-Chromatographic Approach and Zebrafish Model in Bioactivity Screening of Plant Material. Molecules, 30(9), 1979. https://doi.org/10.3390/molecules30091979

Article Metrics

Back to TopTop