Poly(Ethylene Glycol)-b-Poly(D,L-Lactide) Nanoparticles as Potential Carriers for Anticancer Drug Oxaliplatin
Abstract
:1. Introduction
2. Results and Discussion
2.1. Synthesis of mPEG-b-P(D,L)LA Copolymers
2.2. Characterization of Drug-Free mPEG-b-(D,L)LA Nanoparticles
2.3. Characterization of Oxaliplatin-Loaded mPEG-b-(D,L)LA Nanoparticles
3. Materials and Methods
3.1. Materials
3.2. Synthesis of Block Copolymers
3.3. Characterization of mPEG-b-P(D,L)LA Copolymers
3.4. Preparation of mPEG-b-P(D,L)LA Nanoparticles
3.5. Characterization of mPEG-b-P(D,L)LA Nanoparticles
3.6. Evaluation of Drug Loading
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Sample Availability
References
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Sample | Mn1, g/mol | Mn2, g/mol | Mw2, g/mol | PDI 2 |
---|---|---|---|---|
mPEG113-b-P(D,L)LA62 | 9500 | 7300 | 10,500 | 1.4 |
mPEG113-b-P(D,L)LA135 | 14,700 | 9000 | 14,600 | 1.6 |
mPEG113-b-P(D,L)LA173 | 17,500 | 10,400 | 18,000 | 1.7 |
Sample | Rh1, nm | Rg2, nm | Rg3, nm | Dmax/24, nm | R5, nm | 2Rg/Dmax | Rg/R | ζ 6, mV |
---|---|---|---|---|---|---|---|---|
mPEG113-b- P(D,L)LA173 | 28 ± 10 | 11.4 ± 0.1 | 12.5 ± 0.1 | 19.5 ± 1 | 15.4 ± 1 | 0.64 ± 0.05 | 0.81 ± 0.06 | −14 ± 8 |
mPEG113-b- P(D,L)LA135 | 22 ± 10 | 10.1 ± 0.1 | 9.7 ± 0.1 | 17 ± 1 | 11.5 ± 1 | 0.57 ± 0.06 | 0.84 ± 0.09 | −15 ± 4 |
mPEG113-b- P(D,L)LA62 | 16 ± 6 | 9.1 ± 0.1 | 8.6 ± 0.1 | 14 ± 1 | 9 ± 1 | 0.61 ± 0.07 | 0.96 ± 0.11 | −20 ± 4 |
Sample | Rh1, nm | Rg2, nm | Rg3, nm | Dmax/24, nm | R5, nm | 2Rg/Dmax | Rg/R | ζ 6, mV | DLC 7, wt.% |
---|---|---|---|---|---|---|---|---|---|
mPEG113-b- P(D,L)LA173 | 27 ± 10 | 11.1 ± 0.1 | 12.3 ± 0.1 | 19.5 ± 1.0 | 15.3 ± 1.0 | 0.63 ± 0.05 | 0.80 ± 0.07 | −16 ± 9 | 1.5 |
mPEG113-b- P(D,L)LA135 | 25 ± 12 | 9.4 ± 0.1 | 10.4 ± 0.1 | 17.0 ± 1.0 | 12.2 ± 1.0 | 0.61 ± 0.06 | 0.85 ± 0.08 | −17 ± 4 | 2.3 |
mPEG113-b- P(D,L)LA62 | 19 ± 8 | 9.3 ± 0.1 | 8.7 ± 0.1 | 14.0 ± 1.0 | 9.2 ± 1.0 | 0.62 ± 0.07 | 0.95 ± 0.11 | −24 ± 8 | 3.8 |
Sample | Rc1, nm | sint2, nm2/g | σ3,nm−2 | DLC 4, wt.% | EE 5, % |
---|---|---|---|---|---|
mPEG113-b-P(D,L)LA173 | 15.3 | 1.5 × 1020 | 1.0 | 1.5 | 30 |
mPEG113-b-P(D,L)LA135 | 12.2 | 2.0 × 1020 | 0.9 | 2.3 | 46 |
mPEG113-b-P(D,L)LA62 | 9.2 | 2.7 × 1020 | 1.6 | 3.8 | 76 |
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Kadina, Y.A.; Razuvaeva, E.V.; Streltsov, D.R.; Sedush, N.G.; Shtykova, E.V.; Kulebyakina, A.I.; Puchkov, A.A.; Volkov, D.S.; Nazarov, A.A.; Chvalun, S.N. Poly(Ethylene Glycol)-b-Poly(D,L-Lactide) Nanoparticles as Potential Carriers for Anticancer Drug Oxaliplatin. Molecules 2021, 26, 602. https://doi.org/10.3390/molecules26030602
Kadina YA, Razuvaeva EV, Streltsov DR, Sedush NG, Shtykova EV, Kulebyakina AI, Puchkov AA, Volkov DS, Nazarov AA, Chvalun SN. Poly(Ethylene Glycol)-b-Poly(D,L-Lactide) Nanoparticles as Potential Carriers for Anticancer Drug Oxaliplatin. Molecules. 2021; 26(3):602. https://doi.org/10.3390/molecules26030602
Chicago/Turabian StyleKadina, Yulia A., Ekaterina V. Razuvaeva, Dmitry R. Streltsov, Nikita G. Sedush, Eleonora V. Shtykova, Alevtina I. Kulebyakina, Alexander A. Puchkov, Dmitry S. Volkov, Alexey A. Nazarov, and Sergei N. Chvalun. 2021. "Poly(Ethylene Glycol)-b-Poly(D,L-Lactide) Nanoparticles as Potential Carriers for Anticancer Drug Oxaliplatin" Molecules 26, no. 3: 602. https://doi.org/10.3390/molecules26030602