Anti-Fibrotic and Anti-Angiogenic Activities of Osbeckia octandra Leaf Extracts in Thioacetamide-Induced Experimental Liver Cirrhosis
Abstract
:1. Introduction
2. Results
2.1. O. octandra Extracts Prevented Body Weight Loss and Normalized Liver Weight
2.2. O. octandra Extracts Restored Serum Concentrations of Liver Enzymes
2.3. O. octandra Extracts Restored Gross Liver Appearance
2.4. O. octandra Extracts Restore Liver Architecture
2.5. O. octandra Extracts Ameliorate Liver Fibrosis
2.6. O. octandra Extracts Prevent Up-Regulation of Pro-Inflammatory and Profibrotic Cytokine mRNA
2.7. O. octandra Extract Prevents Angiogenesis
3. Discussion
4. Materials and Methods
4.1. Plant Materials
4.2. Preparation of Leaf Extracts
4.3. Experimental Animals
4.4. Body Weight Assessment and Liver Weight Assessment
4.5. Serum Collection and Blood Chemistry
4.6. Tissue Preparation and Histopathology
4.7. RNA Extraction and Real-Time Quantitative Polymerase Chain Reaction (qPCR)
4.8. Assessment of the Effects of Leaf Extract on Angiogenesis In Vitro
4.9. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Sample Availability
References
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Bogahawaththa, S.; Kodithuwakku, S.P.; Wijesundera, K.K.; Siriweera, E.H.; Jayasinghe, L.; Dissanayaka, W.L.; Rajapakse, J.; Herath, C.B.; Tsujita, T.; Wijayagunawardane, M.P.B. Anti-Fibrotic and Anti-Angiogenic Activities of Osbeckia octandra Leaf Extracts in Thioacetamide-Induced Experimental Liver Cirrhosis. Molecules 2021, 26, 4836. https://doi.org/10.3390/molecules26164836
Bogahawaththa S, Kodithuwakku SP, Wijesundera KK, Siriweera EH, Jayasinghe L, Dissanayaka WL, Rajapakse J, Herath CB, Tsujita T, Wijayagunawardane MPB. Anti-Fibrotic and Anti-Angiogenic Activities of Osbeckia octandra Leaf Extracts in Thioacetamide-Induced Experimental Liver Cirrhosis. Molecules. 2021; 26(16):4836. https://doi.org/10.3390/molecules26164836
Chicago/Turabian StyleBogahawaththa, Sudarma, Suranga P. Kodithuwakku, Kavindra K. Wijesundera, Eranga H. Siriweera, Lalith Jayasinghe, Waruna L. Dissanayaka, Jayanthe Rajapakse, Chandana B. Herath, Tadayuki Tsujita, and Missaka P. B. Wijayagunawardane. 2021. "Anti-Fibrotic and Anti-Angiogenic Activities of Osbeckia octandra Leaf Extracts in Thioacetamide-Induced Experimental Liver Cirrhosis" Molecules 26, no. 16: 4836. https://doi.org/10.3390/molecules26164836