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Article

Comparative Protective Effect of Nigella sativa Oil and Vitis vinifera Seed Oil in an Experimental Model of Isoproterenol-Induced Acute Myocardial Ischemia in Rats

1
Department of Pharmacology, Toxicology and Clinical Pharmacology, “Iuliu Hatieganu” University of Medicine and Pharmacy, Victor Babes, No. 8, 400012 Cluj-Napoca, Romania
2
Department of Pathophysiology, Iuliu Haţieganu University of Medicine and Pharmacy Cluj-Napoca, 400012 Cluj-Napoca, Romania
3
“Niculae Stăncioiu” Heart Institute Cluj-Napoca, 19-21 Calea Moților Street, 400001 Cluj-Napoca, Romania
4
Department of Cardiology—Heart Institute, “Iuliu Haţieganu” University of Medicine and Pharmacy Cluj-Napoca, 19-21 Calea Moților Street, 400001 Cluj-Napoca, Romania
5
Department of Family Medicine, Carol Davila University of Medicine and Pharmacy, 050474 Bucharest, Romania
6
Colentina Clinical Hospital, 020125 Bucharest, Romania
7
Research Station for Viticulture and Enology Blaj (SCDVV Blaj), 515400 Blaj, Romania
8
Department of Food Science, University of Agricultural Sciences and Veterinary Medicine of Cluj-Napoca, Calea Manaștur 3-5, 400372 Cluj-Napoca, Romania
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editors: Ada Popolo, Stefan Cristian Vesa and Raffaele Capasso
Molecules 2021, 26(11), 3221; https://doi.org/10.3390/molecules26113221
Received: 29 April 2021 / Revised: 16 May 2021 / Accepted: 24 May 2021 / Published: 27 May 2021
The study’s aim was to characterize the composition of Nigella sativa seed (NSO) and grape seed (GSO) oils, and to evaluate their cardioprotective and anti-inflammatory effect on isoproterenol (ISO)-induced ischemia in rats. Materials and Methods: NSO and GSO supplements were physicochemically characterized. Liquid chromatography–mass spectrometry (HPLC-MS), Fourier-transform infrared spectroscopy (FTIR), and gas chromatography–mass spectrometry (GC-MS) analyses were used to determine the phytochemical composition in the oils. Total polyphenol content (TPC) and in vitro antioxidant activity were also determined. Pretreatment with 4 mL/kg/day NSO or GSO was administered to rats for 14 days. The experimental ischemia was induced by a single administration of ISO 45 mg/kg after 14 days. An electrocardiogram (ECG) was performed initially and 24 h after ISO. Biological evaluation was done at the end of experiment. Results: The HPLC-MS, GC-MS, and FTIR analyses showed that both NSO and GSO are important sources of bioactive compounds, especially catechin and phenolic acids in GSO, while NSO was enriched in flavonoids and thymol derivatives. Pretreatment with GSO and NSO significantly reduced ventricular conduction, prevented the cardiotoxic effect of ISO in ventricular myocardium, and reduced the level of proinflammatory cytokines and CK-Mb. Conclusion: Both NSO and GSO were shown to have an anti-inflammatory and cardioprotective effect in ISO-induced ischemia. View Full-Text
Keywords: black cumin oil; grape seed oil; cardiovascular disease; anti-inflammatory; antioxidant black cumin oil; grape seed oil; cardiovascular disease; anti-inflammatory; antioxidant
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MDPI and ACS Style

Bocsan, I.C.; Pop, R.M.; Sabin, O.; Sarkandy, E.; Boarescu, P.-M.; Roşian, Ş.H.; Leru, P.M.; Chedea, V.S.; Socaci, S.A.; Buzoianu, A.D. Comparative Protective Effect of Nigella sativa Oil and Vitis vinifera Seed Oil in an Experimental Model of Isoproterenol-Induced Acute Myocardial Ischemia in Rats. Molecules 2021, 26, 3221. https://doi.org/10.3390/molecules26113221

AMA Style

Bocsan IC, Pop RM, Sabin O, Sarkandy E, Boarescu P-M, Roşian ŞH, Leru PM, Chedea VS, Socaci SA, Buzoianu AD. Comparative Protective Effect of Nigella sativa Oil and Vitis vinifera Seed Oil in an Experimental Model of Isoproterenol-Induced Acute Myocardial Ischemia in Rats. Molecules. 2021; 26(11):3221. https://doi.org/10.3390/molecules26113221

Chicago/Turabian Style

Bocsan, Ioana Corina, Raluca Maria Pop, Octavia Sabin, Elias Sarkandy, Paul-Mihai Boarescu, Ştefan Horia Roşian, Poliana Mihaela Leru, Veronica Sanda Chedea, Sonia Ancuța Socaci, and Anca Dana Buzoianu. 2021. "Comparative Protective Effect of Nigella sativa Oil and Vitis vinifera Seed Oil in an Experimental Model of Isoproterenol-Induced Acute Myocardial Ischemia in Rats" Molecules 26, no. 11: 3221. https://doi.org/10.3390/molecules26113221

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