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Article

Cell Death Effects Induced by Sulforaphane and Allyl Isothiocyanate on P-Glycoprotein Positive and Negative Variants in L1210 Cells

1
Institute of Molecular Physiology and Genetics, Centre of Biosciences, Slovak Academy of Sciences, Dúbravská cesta 9, 84005 Bratislava, Slovakia
2
Institute of Biochemistry and Microbiology, Faculty of Chemical and Food Technology, Slovak University of Technology in Bratislava, Radlinského 9, 81237 Bratislava, Slovakia
*
Authors to whom correspondence should be addressed.
Academic Editor: Olga Pecháňová
Molecules 2020, 25(9), 2093; https://doi.org/10.3390/molecules25092093
Received: 9 April 2020 / Revised: 24 April 2020 / Accepted: 27 April 2020 / Published: 30 April 2020
(This article belongs to the Special Issue Biological Activity of Natural Substances and Their Derivatives)
Variants of L1210 leukemia cells-namely, parental P-glycoprotein-negative S cells and R and T cells expressing P-glycoprotein, due to selection with vincristine and transfection with the human p-glycoprotein gene, respectively-were used. The responses of these cell variants to two naturally occurring isothiocyanates-sulforaphane (SFN, from cruciferous vegetables) and allyl isothiocyanate (AITC, from mustard, radish, horseradish and wasabi)-were studied. We obtained conflicting results for the cell death effects induced by isothiocyanates, as measured by i. cell counting, which showed inhibited proliferation, and ii. cell metabolic activity via an MTS assay, which showed an increased MTS signal. These results indicated the hyperactivation of cell metabolism induced by treatment with isothiocyanates. In more detailed study, we found that, depending on the cell variants and the isothiocyanate used in treatment, apoptosis and necrosis (detected by annexin-V cells and propidium iodide staining), as well as autophagy (detected with monodansylcadaverine), were involved in cell death. We also determined the cell levels/expression of Bcl-2 and Bax as representative anti- and pro-apoptotic proteins of the Bcl-2 family, the cell levels/expression of members of the canonical and noncanonical NF-κB pathways, and the cell levels of 16 and 18 kDa fragments of LC3B protein as markers of autophagy. View Full-Text
Keywords: P-glycoprotein/ABCB1; multidrug resistance; sulforaphane; allyl isothiocyanate; autophagy; LC3B; NF-κB pathways; apoptosis P-glycoprotein/ABCB1; multidrug resistance; sulforaphane; allyl isothiocyanate; autophagy; LC3B; NF-κB pathways; apoptosis
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MDPI and ACS Style

Kontar, S.; Imrichova, D.; Bertova, A.; Mackova, K.; Poturnayova, A.; Sulova, Z.; Breier, A. Cell Death Effects Induced by Sulforaphane and Allyl Isothiocyanate on P-Glycoprotein Positive and Negative Variants in L1210 Cells. Molecules 2020, 25, 2093. https://doi.org/10.3390/molecules25092093

AMA Style

Kontar S, Imrichova D, Bertova A, Mackova K, Poturnayova A, Sulova Z, Breier A. Cell Death Effects Induced by Sulforaphane and Allyl Isothiocyanate on P-Glycoprotein Positive and Negative Variants in L1210 Cells. Molecules. 2020; 25(9):2093. https://doi.org/10.3390/molecules25092093

Chicago/Turabian Style

Kontar, Szilvia; Imrichova, Denisa; Bertova, Anna; Mackova, Katarina; Poturnayova, Alexandra; Sulova, Zdena; Breier, Albert. 2020. "Cell Death Effects Induced by Sulforaphane and Allyl Isothiocyanate on P-Glycoprotein Positive and Negative Variants in L1210 Cells" Molecules 25, no. 9: 2093. https://doi.org/10.3390/molecules25092093

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