Antibacterial Activity of Honey/Chitosan Nanofibers Loaded with Capsaicin and Gold Nanoparticles for Wound Dressing
Faculty of Biotechnology, Al-Qasim Green University, Babylon 51013, Iraq
Department of Basic Sciences, College of Nursing, University of Misan, Maysan 62001, Iraq
Department of Internal and Preventive Medicine, Veterinary Medicine College, University of Al-Qadisiyah, Al-Diwaniyah 58002, Iraq
Department of Applied Sciences, University of Technology, Baghdad 10066, Iraq
Department of Botany and Microbiology, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia
College of Food and Agriculture Sciences, King Saud University, Riyadh 11451, Saudi Arabia
Faculty of Agriculture, Kafrelsheikh University, Kafr El-Sheikh 33516, Egypt
Authors to whom correspondence should be addressed.
Academic Editors: Jesús Martínez de la Fuente, Laura Asín and Giuseppe De Rosa
Molecules 2020, 25(20), 4770; https://doi.org/10.3390/molecules25204770
Received: 14 August 2020 / Revised: 16 September 2020 / Accepted: 14 October 2020 / Published: 17 October 2020
(This article belongs to the Special Issue Nanoparticles and Their Biological and Biomedical Applications)
This paper describes the preparation, characterization, and evaluation of honey/tripolyphosphate (TPP)/chitosan (HTCs) nanofibers loaded with capsaicin derived from the natural extract of hot pepper (Capsicum annuumL.) and loaded with gold nanoparticles (AuNPs) as biocompatible antimicrobial nanofibrous wound bandages in topical skin treatments. The capsaicin and AuNPs were packed within HTCs in HTCs-capsaicin, HTCs-AuNP, and HTCs-AuNPs/capsaicin nanofibrous mats. In vitro antibacterial testing against Pasteurella multocida, Klebsiella rhinoscleromatis,Staphylococcus pyogenes, and Vibrio vulnificus was conducted in comparison with difloxacin and chloramphenicol antibiotics. Cell viability and proliferation of the developed nanofibers were evaluated using an MTT assay. Finally, in vivo study of the wound-closure process was performed on New Zealand white rabbits. The results indicate that HTCs-capsaicin and HTCs-AuNPs are suitable in inhibiting bacterial growth compared with HTCs and HTCs-capsaicin/AuNP nanofibers and antibiotics (P < 0.01). The MTT assay demonstrates that the nanofibrous mats increased cell proliferation compared with the untreated control (P < 0.01). In vivo results show that the developed mats enhanced the wound-closure rate more effectively than the control samples. The novel nanofibrous wound dressings provide a relatively rapid and efficacious wound-healing ability, making the obtained nanofibers promising candidates for the development of improved bandage materials.