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Article

Infrared Microspectroscopy and Imaging Analysis of Inflammatory and Non-Inflammatory Breast Cancer Cells and Their GAG Secretome

1
Laboratoire de Biochimie Médicale et Biologie Moléculaire, Université de Reims Champagne-Ardenne, 51097 Reims, France
2
CNRS UMR 7369, Matrice Extracellulaire et Dynamique Cellulaire—MEDyC, 51097 Reims, France
3
Zoology Department, Faculty of Science, Cairo University, Giza 12613, Egypt
4
Faculty of Biotechnology, October University for Modern Sciences and Arts, Giza 12613, Egypt
5
Université de Reims Champagne-Ardenne, PICT, 51097 Reims, France
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MIRIAM Beamline B22, Diamond Light Source, Harwell Campus, Chilton-Didcot OX11 0DE, UK
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Department of Gynecology and Obstetrics, Münster University Hospital, 48149 Münster, Germany
8
Université de Reims Champagne-Ardenne, BioSpecT-EA7506, 51097 Reims, France
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editor: Kamilla Malek
Molecules 2020, 25(18), 4300; https://doi.org/10.3390/molecules25184300
Received: 23 July 2020 / Revised: 16 September 2020 / Accepted: 17 September 2020 / Published: 19 September 2020
Glycosaminoglycans (GAGs)/proteoglycans (PGs) play a pivotal role in the metastasis of inflammatory breast cancer (IBC). They represent biomarkers and targets in diagnosis and treatment of different cancers including breast cancer. Thus, GAGs/PGs could represent potential prognostic/diagnostic biomarkers for IBC. In the present study, non-IBC MDA-MB-231, MCF7, SKBR3 cells and IBC SUM149 cells, as well as their GAG secretome were analyzed. The latter was measured in toto as dried drops with high-throughput (HT) Fourier Transform InfraRed (FTIR) spectroscopy and imaging. FTIR imaging was also employed to investigate single whole breast cancer cells while synchrotron-FTIR microspectroscopy was used to specifically target their cytoplasms. Data were analyzed by hierarchical cluster analysis and principal components analysis. Results obtained from HT-FTIR analysis of GAG drops showed that the inter-group variability enabled us to delineate between cell types in the GAG absorption range 1350–800 cm−1. Similar results were obtained for FTIR imaging of GAG extracts and fixed single whole cells. Synchrotron-FTIR data from cytoplasms allowed discrimination between non-IBC and IBC. Thus, by using GAG specific region, not only different breast cancer cell lines could be differentiated, but also non-IBC from IBC cells. This could be a potential diagnostic spectral marker for IBC detection useful for patient management. View Full-Text
Keywords: inflammatory breast cancer; glycosaminoglycans; proteoglycans; secretome; infrared (micro)spectroscopy; imaging; synchrotron-FTIR inflammatory breast cancer; glycosaminoglycans; proteoglycans; secretome; infrared (micro)spectroscopy; imaging; synchrotron-FTIR
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MDPI and ACS Style

Mohamed, H.T.; Untereiner, V.; Cinque, G.; Ibrahim, S.A.; Götte, M.; Nguyen, N.Q.; Rivet, R.; Sockalingum, G.D.; Brézillon, S. Infrared Microspectroscopy and Imaging Analysis of Inflammatory and Non-Inflammatory Breast Cancer Cells and Their GAG Secretome. Molecules 2020, 25, 4300. https://doi.org/10.3390/molecules25184300

AMA Style

Mohamed HT, Untereiner V, Cinque G, Ibrahim SA, Götte M, Nguyen NQ, Rivet R, Sockalingum GD, Brézillon S. Infrared Microspectroscopy and Imaging Analysis of Inflammatory and Non-Inflammatory Breast Cancer Cells and Their GAG Secretome. Molecules. 2020; 25(18):4300. https://doi.org/10.3390/molecules25184300

Chicago/Turabian Style

Mohamed, Hossam T., Valérie Untereiner, Gianfelice Cinque, Sherif A. Ibrahim, Martin Götte, Nguyet Q. Nguyen, Romain Rivet, Ganesh D. Sockalingum, and Stéphane Brézillon. 2020. "Infrared Microspectroscopy and Imaging Analysis of Inflammatory and Non-Inflammatory Breast Cancer Cells and Their GAG Secretome" Molecules 25, no. 18: 4300. https://doi.org/10.3390/molecules25184300

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