Optimization of Extraction Conditions for Gracilaria gracilis Extracts and Their Antioxidative Stability as Part of Microfiber Food Coating Additives
Abstract
:1. Introduction
2. Results and Discussion
2.1. Selective Optimization of Extraction Conditions
2.2. Results of Electrospinning Technique
2.3. Antioxidant Stability of Electrospun Coatings
3. Materials and Methods
3.1. Materials
3.2. Selective Optimization of Extraction Conditions
3.3. Antioxidant Activity Assays
3.4. Electrospinning Technique
3.5. Antioxidant Stability of Electrospun Coatings
4. Conclusions
Author Contributions
Funding
Conflicts of Interest
References
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Sample Availability: Not available. |
Regression Coefficient β | DPPH | TPC | FRAP | Yield |
---|---|---|---|---|
Intercept | 9.057 | 6.642 | −1.220 | 5.077 |
Linear | ||||
Time | 0.43 | 0.006 | −0.256 | 0.012 |
SLR | −0.200 | −0.827 | 0.156 | 1.392 ** |
Ethanol% | −1.744 ** | −2.917 * | −1.594 ** | 1.452 *** |
Quadratic | ||||
Time2 | −0.152 | −0.448 | 0.208 | −0.166 |
SLR2 | 0.298 | 0.509 | −0.310 | −0.717 * |
Ethanol%2 | 2.768 *** | 1.706 | 1.766 ** | −1.702 *** |
CrossProduct | ||||
Time*SLR | −0.005 | −0.018 | −0.014 | 0.137 |
Time*Ethanol% | −0.645 * | 1.137 | 0.326 | 0.014 |
SLR*Ethanol% | −0.463 | 0.366 | 0.556 | −0.653 ** |
R2 | 0.861 | 0.270 | 0.890 | 0.972 |
p value of lack of fit | 0.0011 | 0.0436 | 0.0018 | 0.0090 |
p value of the models | 0.0091 | 0.3207 | 0.0052 | 0.0002 |
DPPH | TPC | FRAP | |
---|---|---|---|
Aqueous; 0.3 mL/h, 14 kV, 10 cm dist. 47% RH; 22 °C | 78.61 ± 6.6 | 63.37 ± 4.2 | 88.89 ± 6.9 |
50% Ethanolic; 0.4 mL/h, 11 kV; 11 cm dist., 46% RH, 21 °C | 100 * | 78.61 ± 2.3 | 80.36 ± 1.1 |
100% Ethanolic; 0.5 mL/h; 11 kV; 11 cm dist., 40% RH, 21 °C | 76.27 ± 2.8 | 90.93 ± 1.1 | 89.9 ± 3.8 |
X1 | X2 | X3 | Ethanol% | Time (min) | SLR (g/mL) |
---|---|---|---|---|---|
−1 | −1 | 0 | 0 | 10 | 1/55 |
−1 | 0 | −1 | 0 | 95 | 1/10 |
−1 | 0 | 1 | 0 | 95 | 1/100 |
−1 | 1 | 0 | 0 | 180 | 1/55 |
1 | −1 | 0 | 100 | 10 | 1/55 |
1 | 0 | −1 | 100 | 95 | 1/10 |
1 | 0 | 1 | 100 | 95 | 1/100 |
1 | 1 | 0 | 100 | 180 | 1/55 |
0 | −1 | −1 | 50 | 10 | 1/10 |
0 | −1 | 1 | 50 | 10 | 1/100 |
0 | 0 | 0 | 50 | 95 | 1/55 |
0 | 0 | 0 | 50 | 95 | 1/55 |
0 | 0 | 0 | 50 | 95 | 1/55 |
0 | 1 | −1 | 50 | 180 | 1/10 |
0 | 1 | 1 | 50 | 180 | 1/100 |
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Reboleira, J.; Ganhão, R.; Mendes, S.; Adão, P.; Andrade, M.; Vilarinho, F.; Sanches-Silva, A.; Sousa, D.; Mateus, A.; Bernardino, S. Optimization of Extraction Conditions for Gracilaria gracilis Extracts and Their Antioxidative Stability as Part of Microfiber Food Coating Additives. Molecules 2020, 25, 4060. https://doi.org/10.3390/molecules25184060
Reboleira J, Ganhão R, Mendes S, Adão P, Andrade M, Vilarinho F, Sanches-Silva A, Sousa D, Mateus A, Bernardino S. Optimization of Extraction Conditions for Gracilaria gracilis Extracts and Their Antioxidative Stability as Part of Microfiber Food Coating Additives. Molecules. 2020; 25(18):4060. https://doi.org/10.3390/molecules25184060
Chicago/Turabian StyleReboleira, João, Rui Ganhão, Susana Mendes, Pedro Adão, Mariana Andrade, Fernanda Vilarinho, Ana Sanches-Silva, Dora Sousa, Artur Mateus, and Susana Bernardino. 2020. "Optimization of Extraction Conditions for Gracilaria gracilis Extracts and Their Antioxidative Stability as Part of Microfiber Food Coating Additives" Molecules 25, no. 18: 4060. https://doi.org/10.3390/molecules25184060