Next Article in Journal
Anthocyanins: A Comprehensive Review of Their Chemical Properties and Health Effects on Cardiovascular and Neurodegenerative Diseases
Next Article in Special Issue
Biochemical and Computational Approach of Selected Phytocompounds from Tinospora crispa in the Management of COVID-19
Previous Article in Journal
Health Benefits of Plant-Derived Sulfur Compounds, Glucosinolates, and Organosulfur Compounds
Previous Article in Special Issue
GC-MS Phytochemical Profiling, Pharmacological Properties, and In Silico Studies of Chukrasia velutina Leaves: A Novel Source for Bioactive Agents
Open AccessArticle

Workflow for the Quantification of Soluble and Insoluble Carbohydrates in Soybean Seed

1
BioDiscovery Institute, University of North Texas, Denton, TX 76201, USA
2
Crop, Soil and Environmental Sciences, University of Arkansas, Fayetteville, AR 72701, USA
3
Fisher Delta Research Center, University of Missouri, Portageville, MO 63873, USA
4
USDA-ARS, Soybean & Nitrogen Fixation Unit, Raleigh, NC 27607, USA
5
Department of Horticulture and Crop Science, The Ohio State University, Columbus, OH 43210, USA
*
Author to whom correspondence should be addressed.
Academic Editor: Jesus Simal-Gandara
Molecules 2020, 25(17), 3806; https://doi.org/10.3390/molecules25173806
Received: 30 July 2020 / Revised: 18 August 2020 / Accepted: 20 August 2020 / Published: 21 August 2020
(This article belongs to the Special Issue Modern Analytical Techniques in Metabolomics Analysis)
Soybean seed composition has a profound impact on its market value and commercial use as an important commodity. Increases in oil and protein content have been historically pursued by breeders and genetic engineers; consequently, rapid methods for their quantification are well established. The interest in complete carbohydrate profiles in mature seeds, on the other hand, has recently increased due to numerous attempts to redirect carbohydrates into oil and protein or to offer specialty seed with a specific sugar profile to meet animal nutritional requirements. In this work, a sequential protocol for quantifying reserve and structural carbohydrates in soybean seed was developed and validated. Through this procedure, the concentrations of soluble sugars, sugar alcohols, starch, hemicellulose, and crystalline cellulose can be determined in successive steps from the same starting material using colorimetric assays, LC–MS/MS, and GC–MS. The entire workflow was evaluated using internal standards to estimate the recovery efficiency. Finally, it was successfully applied to eight soybean genotypes harvested from two locations, and the resulting correlations of carbohydrate and oil or protein are presented. This methodology has the potential not only to guide soybean cultivar optimization processes but also to be expanded to other crops with only slight modifications. View Full-Text
Keywords: carbohydrates; soybean; oil; protein; soluble sugars; starch; matrix polysaccharide; crystalline cellulose; Glycine max carbohydrates; soybean; oil; protein; soluble sugars; starch; matrix polysaccharide; crystalline cellulose; Glycine max
Show Figures

Graphical abstract

MDPI and ACS Style

Moretti, A.; Arias, C.L.; Mozzoni, L.A.; Chen, P.; McNeece, B.T.; Mian, M.A.R.; McHale, L.K.; Alonso, A.P. Workflow for the Quantification of Soluble and Insoluble Carbohydrates in Soybean Seed. Molecules 2020, 25, 3806.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop