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Open AccessArticle

Extraction and Determination of Polar Bioactive Compounds from Alfalfa (Medicago sativa L.) Using Supercritical Techniques

1
Łukasiewicz Research Network–New Chemical Syntheses Institute, 24–110 Puławy, Poland
2
Department of Environmental Chemistry and Bioanalytics, Faculty of Chemistry, Nicolaus Copernicus University, 87–100 Toruń, Poland
3
Interdisciplinary Centre of Modern Technologies, Nicolaus Copernicus University, 87–100 Toruń, Poland
*
Author to whom correspondence should be addressed.
Molecules 2019, 24(24), 4608; https://doi.org/10.3390/molecules24244608
Received: 20 November 2019 / Revised: 12 December 2019 / Accepted: 15 December 2019 / Published: 16 December 2019
The aim of this research was to select parameters for supercritical extraction with CO2 of Medicago sativa L., considered as functional food, in quarter-technical plant, providing the highest concentration of bioactive polar constituents and simultaneously maintaining the highest efficiency of the process. For the purpose of optimization, mathematical statistics was used. Qualitative analysis of products was performed with supercritical fluid chromatography (SFC). The SFC analysis revealed a proper separation of flavonoids and phenolics acids for dedicated TFC and TPC optimal parameters. The obtained results have proved that it is a possibility to extract polar compounds with non-polar solvent under higher values of pressure and temperature and to enrich product with desired group of bioactive compounds with proper optimization. The proposed extraction technique allows to obtain on an industrial scale, using an environmentally friendly solvent, a preparation rich in biologically active nutrients that can be implemented in the cosmetics, pharmaceutical and food industries. View Full-Text
Keywords: supercritical fluid extraction; SFE; supercritical fluid chromatography; SFC; bioactive compounds; Medicago sativa L.; response surface methodology; RSM; quarter-technical plant; flavonoids; phenolics compounds supercritical fluid extraction; SFE; supercritical fluid chromatography; SFC; bioactive compounds; Medicago sativa L.; response surface methodology; RSM; quarter-technical plant; flavonoids; phenolics compounds
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Wrona, O.; Rafińska, K.; Walczak-Skierska, J.; Możeński, C.; Buszewski, B. Extraction and Determination of Polar Bioactive Compounds from Alfalfa (Medicago sativa L.) Using Supercritical Techniques. Molecules 2019, 24, 4608.

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