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Open AccessArticle

Applicability of a Monolithic Column for Separation of Isoquinoline Alkalodis from Chelidonium majus Extract

1
Department of Analytical Chemistry, Medical University of Lublin, 20-093 Lublin, Poland
2
Department of Plant Physiology and Biophysics, Maria Curie-Skłodowska University, 20-033 Lublin, Poland
3
Faculty of Chemistry, Maria Curie-Skłodowska University, 20-031 Lublin, Poland
*
Author to whom correspondence should be addressed.
Molecules 2019, 24(19), 3612; https://doi.org/10.3390/molecules24193612
Received: 6 September 2019 / Revised: 26 September 2019 / Accepted: 5 October 2019 / Published: 7 October 2019
(This article belongs to the Special Issue Chromatographic Science of Natural Products)
Isoquinoline alkaloids are the main group of secondary metabolites present in Chelidonium majus extracts, and they are still the object of interest of many researchers. Therefore, the development of methods for the investigation and separation of the alkaloids is still an important task. In this work, the application potential of a silica-based monolithic column for the separation of alkaloids was assessed. The influence of the organic modifier, temperature, salt concentration, and pH of the eluent on basic chromatographic parameters such as retention, resolution between neighboring peaks, chromatographic plate numbers, and peak asymmetry were investigated. Based on the obtained results, a gradient elution program was developed and used to separate and quantitatively determine the main alkaloids in a Chelidonium majus root extract. View Full-Text
Keywords: Alkaloids; isoquinoline alkaloids; monolithic columns; Chelidonium majus; HPLC Alkaloids; isoquinoline alkaloids; monolithic columns; Chelidonium majus; HPLC
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Staniak, M.; Wójciak-Kosior, M.; Sowa, I.; Strzemski, M.; Sawicki, J.; Dresler, S.; Tyszczuk-Rotko, K. Applicability of a Monolithic Column for Separation of Isoquinoline Alkalodis from Chelidonium majus Extract. Molecules 2019, 24, 3612.

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